Abstract | BACKGROUND AND OBJECTIVES: In our laboratory, we have developed a new model of carcinoma of the bile duct in Syrian golden hamsters, using N-nitrosobis(2-oxopropyl)amine (BOP). Morphologic and biologic characteristics of the carcinoma induced in this model are similar to those seen in humans. In order to examine the gene-related carcinogenesis of intrahepatic bile duct carcinoma, we investigated mutations in the K-ras gene in various early hyperplastic and neoplastic lesions of these hamsters, according to the original sites of the lesions. METHODS: Inbred female hamsters were given a subcutaneous injection of N-nitrosobis(2-oxopropyl)amine (BOP) following dissection of the extrahepatic bile duct on the distal end of the common duct and preparation of a cholecystoduodenostomy (CDDB) or simple laparotomy (SL). Neoplastic lesions arising from the intrahepatic bile duct were histologically examined, and K-ras mutations were investigated. RESULTS: CONCLUSIONS: A part of the hyperplastic lesions of the intrahepatic bile duct presented K-ras gene mutation. This suggests that K-ras gene mutation is an early event in the carcinogenic process. In carcinoma of the intrahepatic bile duct, the lesion arising from a large bile duct of the hepatic hilum tended to exhibit a higher frequency of K-ras gene mutation than did a tubular lesion arising from ductule or ductal proliferation. This hamster model is useful to examine the carcinogenesis of human intrahepatic bile duct carcinoma.
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Authors | S Yamanaka, T Tomioka, Y Tajima, K Okada, H Shiku, T Kanematsu |
Journal | Journal of surgical oncology
(J Surg Oncol)
Vol. 66
Issue 2
Pg. 97-103
(Oct 1997)
ISSN: 0022-4790 [Print] United States |
PMID | 9354164
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Carcinogens
- Nitrosamines
- nitrosobis(2-oxopropyl)amine
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Topics |
- Adenocarcinoma
(chemically induced, genetics)
- Adenocarcinoma, Papillary
(chemically induced, genetics)
- Animals
- Bile Duct Neoplasms
(chemically induced, genetics)
- Bile Ducts, Intrahepatic
- Carcinogens
- Cricetinae
- Female
- Genes, ras
(genetics)
- Mesocricetus
- Mutation
- Nitrosamines
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