Most primary cutaneous CD30-positive lymphoproliferative disorders have a CD4-positive cytotoxic T-cell phenotype.

Abstract

Primary cutaneous CD30-positive (anaplastic) large T-cell lymphomas and lymphomatoid papulosis are closely related types of cutaneous T-cell lymphoma with a favorable prognosis. The neoplastic T cells in these conditions have the phenotype of activated CD3+, CD4+, CD8-, CD30+ skin homing T cells, but their normal counterpart has not yet been defined. To further characterize the cellular origin of the neoplastic T cells, skin biopsies from 14 patients with primary cutaneous CD30-positive (anaplastic) large T-cell lymphomas, nine patients with lymphomatoid papulosis, and six patients with primary cutaneous CD30-negative pleomorphic large T-cell lymphomas were stained with monoclonal antibodies against cytotoxic cell-associated molecules granzyme B and T-cell restricted intracellular antigen. In nine of nine lymphomatoid papulosis and in 10 of 14 CD30-positive primary cutaneous large T-cell lymphomas, expression of granzyme B and T-cell restricted intracellular antigen by variable numbers of neoplastic cells was found. Expression of granzyme B by the neoplastic CD30-positive T cells was confirmed by double-staining for granzyme B and CD30 (three cases) and by the detection of granzyme B mRNA using RNA in situ hybridization (one case). In most cases equal numbers of granzyme-B-positive and T-cell restricted intracellular antigen positive tumor cells were observed. In five of six CD30-negative primary cutaneous large T-cell lymphomas the neoplastic cells did not express these proteins, whereas in one case a sporadic positive tumor cell was found. These results demonstrate that, in contrast to primary cutaneous CD30-negative pleomorphic large T-cell lymphomas, the neoplastic cells in most primary cutaneous CD30-positive (anaplastic) large T-cell lymphomas and lymphomatoid papulosis have a unique CD4+, CD8-, cytotoxic T-cell phenotype.

• Authors: J A Kummer, M H Vermeer, D Dukers, C J Meijer, R Willemze
• Journal: The Journal of investigative dermatology (J Invest Dermatol) Vol. 109 Issue 5 Pg. 636-40 (Nov 1997) ISSN: 0022-202X [Print] United States
• PMID: 9347791 (Publication Type: Journal Article)

Chemical References

• Ki-1 Antigen
• Membrane Proteins
• Poly(A)-Binding Proteins
• Proteins
• RNA-Binding Proteins
• T-Cell Intracellular Antigen-1
• TIA1 protein, human
• Granulocyte Colony-Stimulating Factor
• GZMB protein, human
• Granzymes
• Serine Endopeptidases

Topics

• CD4-Positive T-Lymphocytes (metabolism)
• Granulocyte Colony-Stimulating Factor (biosynthesis)
• Granzymes
• Humans
• Ki-1 Antigen (analysis)
• Lymphoma, Large B-Cell, Diffuse (genetics, immunology)
• Lymphoma, T-Cell, Cutaneous (genetics, immunology)
• Lymphoproliferative Disorders (genetics, immunology)
• Membrane Proteins (biosynthesis)
• Phenotype
• Poly(A)-Binding Proteins
• Proteins
• RNA-Binding Proteins (biosynthesis)
• Serine Endopeptidases (biosynthesis)
• T-Cell Intracellular Antigen-1