Overexpression of cyclin E in acute myelogenous leukemia.

Abstract

Cyclin E is one of the G1 cyclins that play an important role in cell proliferation. Overexpression of cyclin E protein has been reported in several solid tumors, but little is known about the involvement of cyclin E in leukemia. In this study, we analyzed the expression of cyclin E gene product in 85 patients with acute myelogenous leukemia (AML) by Western blot analysis. In 23 of 85 AML samples (27%), cyclin E expression was enhanced in blasts. Among the French-American-British classification of AML, the ratio of the samples with enhanced cyclin E expression was high in M5 and low in M2 and M3. No rearrangements were observed by Southern blot analysis in these AML blasts with enhanced cyclin E expression. Flow cytometric analysis showed no correlation between overexpression of cyclin E and cell cycle distribution. Immunoblot analysis of cyclin D1 showed no correlation between overexpression of cyclin E and that of cyclin D1. Interestingly, p27 expression detected by Western blotting was apparently enhanced in 18 of 23 AML cells with enhanced cyclin E expression but none of 14 AML cells without enhanced cyclin E exhibited enhanced p27 expression. The rates of complete remission and of disease-free survival of the patients with M4 or M5 leukemia blasts with overexpressed cyclin E seemed to be low. Therefore, we suggest the necessity of a larger-scale study to elucidate the contribution of cyclin E overexpression to the phenotype and the prognosis of certain AML.

• Authors: H Iida, M Towatari, M Tanimoto, Y Morishita, Y Kodera, H Saito
• Journal: Blood (Blood) Vol. 90 Issue 9 Pg. 3707-13 (Nov 01 1997) ISSN: 0006-4971 [Print] United States
• PMID: 9345056 (Publication Type: Journal Article)

Chemical References

• Cyclin E

Topics

• Adult
• Aged
• Blotting, Southern
• Blotting, Western
• Cyclin E (biosynthesis)
• Flow Cytometry
• Gene Expression Regulation, Neoplastic
• Humans
• Leukemia, Myeloid, Acute (metabolism)
• Male
• Middle Aged