Infection with many viruses results in the selective shutoff of host
protein synthesis. A common target for virus interference with host
protein synthesis is the
cap-binding protein complex,
eIF4F. The large subunit of the complex,
eIF4G, is cleaved upon picornavirus (except
cardiovirus) infection.
Infection with adenovirus and influenza virus causes dephosphorylation of the cap-binding subunit,
eIF4E. Recently, it has been shown that
infection with poliovirus or encephalomyocarditis virus activates 4E-BP1, which is a specific inhibitor of
eIF4E. Here we show that early in
adenovirus infection, 4E-BP1 and its related
protein 4E-BP2 are phosphorylated and hence inactivated. This is not consistent with a role of 4E-BPs in adenovirus-induced shutoff, but could explain the increase in
protein synthesis reported early in
infection. Phosphorylation of 4E-BP1 and 4E-BP2 is consistent with earlier findings in adenovirus-infected cells on the activation of the
protein kinase p70(S6k), whose phosphorylation lies on the same pathway as 4E-BPs, by E1A. Findings similar to those described here were reported for 4E-BP1 by D. Feigenblum and R. J. Schneider (1996, Mol. Cell. Biol. 16, 5450-5457).