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Comparative immunoreactivity of CD-68 and HMB-45 in malignant melanoma, neural tumors and nevi.

Abstract
The monoclonal antibody CD 68 (KP 1) reacts with fibrohistiocytic and some epithelial neoplasms; its reactivity compared with that of HMB 45 in malignant melanoma (MM) and neural tumors needs further elucidation. Using a streptavidin-biotin-immunoperoxidase procedure, we examined the reactivity of 65 MM (46 conventional, 1 polypoid, 6 desmoplastic [DMM], and 12 metastatic), 21 neurofibromas, 1 neurofibrosarcoma, 10 schwannomas, 1 perineurioma, 2 neurothekeomas, and 14 blue and 26 other nevi for CD-68, HMB-45-defined antigen, S 100 and neurofilament protein. A positive staining for CD 68 was observed in 38 of 42 primary, 5 of 6 DMM, and 11 of 12 metastatic melanomas; 6 of 10 schwannomas; 5 of 10 nevi with junctional component and all 14 blue nevi. All 21 neurofibromas, 1 each neurofibrosarcoma and perineurioma, both neurothekeomas, and all 12 nevi with dermal component were CD 68-negative. HBM 45 was expressed by all 44 primary, none of 6 DMM, and 7 of 12 metastatic melanomas; by none of 10 schwannomas, 6 neurofibromas, 1 neurofibrosarcoma, 1 perineurioma and 2 neurothekeomas. Both junctional nevi, 8 of 10 nevi with junctional components, 1 of 10 dermal components of junctional nevi, and 11 of 13 blue nevi were also HMB 45 positive. Except for 1 perineurioma, S 100 decorated all tumors examined. NF was immunoreactive in 1 of 45 conventional melanomas, 2 of 21 neurofibromas, 2 of 10 schwannomas, and 3 of 10 blue nevi; it was non-reactive in all polypoid, desmoplastic and metastatic melanomas; neurofibrosarcoma, perineurioma, neurothekeoma and other nevi. We conclude that the CD-68-reactivity in primary melanomas, neurofibromas, neurofibrosarcomas, perineuriomas, and nevi was similar to that of HMB 45. The significantly higher CD 68-positivity than of HMB 45 in metastatic and desmoplastic melanomas and schwannomas may be of diagnostic value.
AuthorsI A Shah, O S Gani, L Wheler
JournalPathology, research and practice (Pathol Res Pract) Vol. 193 Issue 7 Pg. 497-502 ( 1997) ISSN: 0344-0338 [Print] Germany
PMID9342756 (Publication Type: Journal Article)
Chemical References
  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • Antigens, Neoplasm
  • Biomarkers, Tumor
  • CD68 antigen, human
  • Melanoma-Specific Antigens
  • Neoplasm Proteins
  • Neurofilament Proteins
  • S100 Proteins
Topics
  • Antigens, CD (analysis)
  • Antigens, Differentiation, Myelomonocytic (analysis)
  • Antigens, Neoplasm (analysis)
  • Biomarkers, Tumor (analysis)
  • Humans
  • Immunohistochemistry
  • Melanoma (chemistry, immunology)
  • Melanoma-Specific Antigens
  • Neoplasm Proteins (analysis)
  • Neoplasms, Nerve Tissue (chemistry)
  • Neurilemmoma (chemistry)
  • Neurofibroma (chemistry)
  • Neurofilament Proteins (analysis)
  • Nevus (chemistry)
  • S100 Proteins (analysis)

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