Abstract | BACKGROUND: METHODS AND RESULTS: We generated recombinant adenoviral vectors containing HSP60, HSP10, or a combination of the two genes. These adenoviral constructs overexpress significant amounts of these stress proteins in both rat neonatal cardiomyocytes and the myogenic H9 c2 cell line. Cells infected with an adenoviral construct overexpressing both HSP60 and HSP10 were found to be protected against simulated ischemia, whereas cells infected with adenoviral constructs overexpressing only HSP60 or HSP10 alone were not rendered tolerant to simulated ischemic injury. CONCLUSIONS:
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Authors | S Lau, N Patnaik, M R Sayen, R Mestril |
Journal | Circulation
(Circulation)
Vol. 96
Issue 7
Pg. 2287-94
(Oct 07 1997)
ISSN: 0009-7322 [Print] United States |
PMID | 9337202
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Chaperonin 10
- Chaperonin 60
- Recombinant Proteins
- Methionine
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Topics |
- Adenoviridae
- Animals
- Animals, Newborn
- Cell Line
- Chaperonin 10
(biosynthesis, isolation & purification)
- Chaperonin 60
(biosynthesis, isolation & purification)
- Genetic Vectors
- Humans
- Methionine
(metabolism)
- Mice
- Mice, Transgenic
- Myocardial Reperfusion Injury
(metabolism, prevention & control)
- Myocardium
(metabolism)
- Rats
- Recombinant Proteins
(biosynthesis, isolation & purification)
- Transfection
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