HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

IL-12 induces IFN-gamma expression and secretion in mouse peritoneal macrophages.

Abstract
We previously reported that resting mouse peritoneal macrophages (PM) constitutively express low levels of IFN-gamma, whose production is consistently enhanced by exogenous IFN-gamma. In this study, we investigated the effects of IL-12 on the replication of vesicular stomatitis virus and on IFN-gamma gene expression in mouse PM. The addition of IL-12 to freshly explanted PM resulted in the persistence of an antiviral state to vesicular stomatitis virus, while control PM progressively became permissive for virus replication after 3 to 4 days in culture. The IL-12-induced antiviral state was inhibited by Abs to IFN-gamma, suggesting that endogenous IFN-gamma was largely responsible for this antiviral response. Moreover, IL-12 induced a consistent secretion of IFN-gamma, especially in cultured PM. The IL-1 2-induced antiviral state and IFN-gamma production were observed using PM from various strains of mice, including LPS-defective C3H/HeJ, NK-deficient bg/bg, DBA/2, Swiss (CD1), and Swiss nude mice treated or not with anti-asialo GM1 Abs. A 4-h treatment with IL-12 was sufficient to induce a marked accumulation of IFN-gamma mRNA, which was greater in cultured PM than in freshly harvested cells. Lastly, immunofluorescence studies in IL-12-stimulated macrophages clearly showed an enhancement of immunoreactive IFN-gamma compared with basal levels in cells exhibiting a macrophage (i.e., F4/80-positive) phenotype. Together, these findings demonstrate that IL-12 can directly stimulate mouse PM to produce IFN-gamma. We suggest that IL-12-induced IFN-gamma production by macrophages can play some role in the generation of the antiviral and immunoregulatory effects of IL-12.
AuthorsP Puddu, L Fantuzzi, P Borghi, B Varano, G Rainaldi, E Guillemard, W Malorni, P Nicaise, S F Wolf, F Belardelli, S Gessani
JournalJournal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 159 Issue 7 Pg. 3490-7 (Oct 1 1997) ISSN: 0022-1767 [Print] UNITED STATES
PMID9317148 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies
  • Antigens, Differentiation
  • Antiviral Agents
  • RNA, Messenger
  • monocyte-macrophage differentiation antigen
  • Interleukin-12
  • G(M1) Ganglioside
  • asialo GM1 ganglioside
  • Interferon-gamma
Topics
  • Animals
  • Antibodies (pharmacology)
  • Antigens, Differentiation (analysis)
  • Antiviral Agents (pharmacology)
  • Cells, Cultured
  • G(M1) Ganglioside (immunology)
  • Interferon-gamma (biosynthesis, genetics, physiology, secretion)
  • Interleukin-12 (pharmacology)
  • Intracellular Fluid (immunology)
  • Macrophages, Peritoneal (immunology, secretion, virology)
  • Male
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred DBA
  • Mice, Nude
  • RNA, Messenger (biosynthesis, drug effects)
  • Species Specificity
  • Tumor Cells, Cultured
  • Vesicular stomatitis Indiana virus (drug effects, immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: