Abstract |
Multiparous Holstein cows were infected in two quarters by intramammary infusion with Streptococcus agalactiae and slaughtered approximately 36 h later. Mammary tissue was removed from the infected quarters, uninfected contralateral quarters, and from pair-slaughtered uninfected controls; the tissue was frozen in liquid nitrogen. The RNA was extracted, and Northern blot analysis was performed for a variety of growth factors, stress-induced genes, milk protein genes, and control genes. Infection increased levels of mRNA coding for heat shock proteins 89 alpha, 89 beta, 70, 60, and 27. Simultaneously, concentrations of alpha-lactalbumin and casein mRNA decreased; alpha-lactalbumin mRNA showed a greater decline. The mRNA for several growth factors, including acidic fibroblast growth factor, basic fibroblast growth factor, epidermal growth factor, transforming growth factor-alpha, IGF-I, and IGF-II, were also increased as was the apoptosis marker, testosterone-repressed prostate mucin-2. Concentrations of mRNA for controls, beta-actin, and glyceraldehyde-3-phosphate dehydrogenase were unaffected. These results indicate that mastitis induces changes in the levels of mRNA encoding for a variety of peptide growth factors. Such changes in growth factors could be important in a variety of processes that occur during infection, such as protection against injury or tissue repair and recovery processes.
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Authors | L G Sheffield |
Journal | Journal of dairy science
(J Dairy Sci)
Vol. 80
Issue 9
Pg. 2020-4
(Sep 1997)
ISSN: 0022-0302 [Print] United States |
PMID | 9313143
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Caseins
- Growth Substances
- RNA, Messenger
- Transforming Growth Factor alpha
- Fibroblast Growth Factor 2
- Fibroblast Growth Factor 1
- Epidermal Growth Factor
- Insulin-Like Growth Factor I
- Insulin-Like Growth Factor II
- Lactalbumin
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Topics |
- Animals
- Caseins
(genetics)
- Cattle
- Epidermal Growth Factor
(genetics)
- Female
- Fibroblast Growth Factor 1
(genetics)
- Fibroblast Growth Factor 2
(genetics)
- Growth Substances
(genetics)
- Insulin-Like Growth Factor I
(genetics)
- Insulin-Like Growth Factor II
(genetics)
- Lactalbumin
(genetics)
- Mammary Glands, Animal
(metabolism)
- Mastitis, Bovine
(metabolism)
- RNA, Messenger
(metabolism)
- Transforming Growth Factor alpha
(genetics)
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