Mastitis increases growth factor messenger ribonucleic acid in bovine mammary glands.

Abstract

Multiparous Holstein cows were infected in two quarters by intramammary infusion with Streptococcus agalactiae and slaughtered approximately 36 h later. Mammary tissue was removed from the infected quarters, uninfected contralateral quarters, and from pair-slaughtered uninfected controls; the tissue was frozen in liquid nitrogen. The RNA was extracted, and Northern blot analysis was performed for a variety of growth factors, stress-induced genes, milk protein genes, and control genes. Infection increased levels of mRNA coding for heat shock proteins 89 alpha, 89 beta, 70, 60, and 27. Simultaneously, concentrations of alpha-lactalbumin and casein mRNA decreased; alpha-lactalbumin mRNA showed a greater decline. The mRNA for several growth factors, including acidic fibroblast growth factor, basic fibroblast growth factor, epidermal growth factor, transforming growth factor-alpha, IGF-I, and IGF-II, were also increased as was the apoptosis marker, testosterone-repressed prostate mucin-2. Concentrations of mRNA for controls, beta-actin, and glyceraldehyde-3-phosphate dehydrogenase were unaffected. These results indicate that mastitis induces changes in the levels of mRNA encoding for a variety of peptide growth factors. Such changes in growth factors could be important in a variety of processes that occur during infection, such as protection against injury or tissue repair and recovery processes.

• Authors: L G Sheffield
• Journal: Journal of dairy science (J Dairy Sci) Vol. 80 Issue 9 Pg. 2020-4 (Sep 1997) ISSN: 0022-0302 [Print] United States
• PMID: 9313143 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Caseins
• Growth Substances
• RNA, Messenger
• Transforming Growth Factor alpha
• Fibroblast Growth Factor 2
• Fibroblast Growth Factor 1
• Epidermal Growth Factor
• Insulin-Like Growth Factor I
• Insulin-Like Growth Factor II
• Lactalbumin

Topics

• Animals
• Caseins (genetics)
• Cattle
• Epidermal Growth Factor (genetics)
• Female
• Fibroblast Growth Factor 1 (genetics)
• Fibroblast Growth Factor 2 (genetics)
• Growth Substances (genetics)
• Insulin-Like Growth Factor I (genetics)
• Insulin-Like Growth Factor II (genetics)
• Lactalbumin (genetics)
• Mammary Glands, Animal (metabolism)
• Mastitis, Bovine (metabolism)
• RNA, Messenger (metabolism)
• Transforming Growth Factor alpha (genetics)