Abstract | AIM: METHODS: The protein and mRNA levels of the Ewing's sarcoma cell line RM 82 were examined in vitro with "Enhanced Amplified Sensitivity Immunoassay" (EASIA) and semiquantitative RT-PCR before and after treatment with single doses of 2 to 40 Gy, 1 to 72 hours after irradiation. After successful transplantation to nude mice, the time and dose correlation of TNF alpha mRNA production was examined in vivo. RESULTS: In vitro, RM 82 had a basal protein level of TNF alpha of 20.1 +/- 4.3 pg/ml/10(6) cells. We observed a time- and dose-dependent increase of TNF alpha expression with a maximum of 125 pg/ml/10(6) (5.9 fold) 24 hours after irradiation with 20 Gy. At the mRNA level, the maximal up-regulation occurred 6 to 12 hours after 10 Gy. In vivo, the xenograft tumor maintained the capacity of TNF alpha expression. Time- and dose-dependency in mRNA production showed a maximum increase 6 hours after treatment with 10 Gy. CONCLUSIONS: The presented experiments show in vitro a dose- and time-dependent up-regulation of TNF alpha in the Ewing's sarcoma cell line RM 82 on protein and mRNA level. For the first time this phenomenon was also observed in vivo in a human xenograft tumor. This tumor model could be used for further experiments to examine the role of TNF alpha as a biologic radiation response modifier in human tumors.
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Authors | C Rübe, C Finke, F van Valen, K L Schäfer, B Dockhorn-Dworniczak, N Willich |
Journal | Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]
(Strahlenther Onkol)
Vol. 173
Issue 8
Pg. 407-14
(Aug 1997)
ISSN: 0179-7158 [Print] Germany |
Vernacular Title | Nachweis einer strahleninduzierten Produktion von Tumor-Nekrose-Faktor alpha im Ewing-Sarkom RM 82 in vitro und in vivo. |
PMID | 9289857
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- DNA Primers
- RNA, Messenger
- Tumor Necrosis Factor-alpha
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Topics |
- Animals
- Base Sequence
- DNA Primers
- Dose-Response Relationship, Radiation
- Electrons
- Female
- Gamma Rays
- Humans
- Male
- Mice
- Mice, Nude
- Molecular Sequence Data
- Neoplasm Transplantation
- Polymerase Chain Reaction
(instrumentation, methods)
- RNA, Messenger
(analysis, biosynthesis, radiation effects)
- Sarcoma, Ewing
(metabolism, radiotherapy)
- Time Factors
- Transplantation, Heterologous
- Tumor Cells, Cultured
- Tumor Necrosis Factor-alpha
(analysis, biosynthesis, radiation effects)
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