Human endometriosis-derived permanent cell line (FbEM-1): establishment and characterization.

Abstract

A human epithelial-like cell line derived from peritoneal implants from a patient with gonadotrophin-releasing hormone (GnRH) agonist-resistant endometriosis graded as stage IVd according to the American Fertility Society classification was established in vitro. This cell line, designated FbEM-1, exhibited an epithelial-like morphology, grew in suspension and was immunoreactive for cytokeratins 8, 18, 19, vimentin and human leukocyte class I antigens. The cultured cells were negative for various haematopoietic cell markers, including the lymphoid cell antigens CD3, CD20 and CD45, von Willebrandt factor, carcinoembryonic antigen and the carcinoma antigen-125 (CA-125). In addition, the FbEM-1 cells were found to be moderately positive for periodic acid Schiff's (PAS) solution but were negative for alpha-naphthyl acetate esterase, peroxidase and chloroacetate esterase activities. Using specific antibodies against the progesterone, androgen and oestrogen receptors, approximately 40% and 5-10% of the cells immunostained for progesterone and androgen receptors respectively, while oestrogen receptors were not detected. On cytogenetic analysis using R-banding, these cells showed numerous chromosomal aberrations, including loss of one chromosome X, 4q+, 5q+, trisomy 7,8 and 10 and tetrasomy of chromosomes 17, 18, 19 and 20. These data show that the continuously growing FbEM-1 cell line established from endometriotic implants may be useful in achieving better understanding of the histogenesis of endometriosis.

• Authors: J Bouquet de Jolinière, P Validire, M Canis, M Doussau, M Levardon, J Gogusev
• Journal: Human reproduction update (Hum Reprod Update) 1997 Mar-Apr Vol. 3 Issue 2 Pg. 117-23 ISSN: 1355-4786 [Print] England
• PMID: 9286736 (Publication Type: Journal Article)

Chemical References

• Antigens, Surface
• Histocompatibility Antigens Class I
• Receptors, Androgen
• Receptors, Progesterone
• Vimentin
• Keratins

Topics

• Animals
• Antigens, Surface (analysis)
• Cell Division
• Cell Line
• Cell Transplantation
• Chromosome Aberrations
• Endometriosis (genetics, metabolism, pathology)
• Female
• Histocompatibility Antigens Class I (analysis)
• Humans
• Immunohistochemistry
• Karyotyping
• Keratins (analysis)
• Mice
• Mice, Nude
• Periodic Acid-Schiff Reaction
• Receptors, Androgen (analysis)
• Receptors, Progesterone (analysis)
• Vimentin (analysis)