The
biological roles of the soluble
granulocyte colony-stimulating factor (
G-CSF) receptor, which arises as a result of alternative RNA splicing, are as yet unknown. In this study, we examined the in vitro effect of a chimeric
protein composed of the extracellular region of a murine
G-CSF receptor and the human
IgG1 Fc region because a human natural soluble
G-CSF receptor was not available. First, we found that this chimeric soluble
G-CSF receptor could inhibit the
biological activity of
G-CSF on normal bone marrow colony formation. Because
G-CSF also plays an important role in the proliferation of leukemic blast cells, we next examined the effect of the soluble
G-CSF receptor on leukemic blast colony formation in 10
acute myeloblastic leukemia cases. Although
G-CSF stimulated the proliferation of leukemic progenitor cells to form leukemic blast colonies, the chimeric soluble
G-CSF receptor completely inhibited this stimulatory effect. Furthermore, the chimeric soluble
G-CSF receptor also inhibited spontaneous leukemic blast colony formation in two cases. Because a high concentration of
G-CSF was observed in the supernatants of leukemic blast cells from these two cases, it seems likely that the soluble
G-CSF receptor cut off the autocrine growth mechanism of leukemic blast cells mediated by
G-CSF. These findings suggest the possibility that the soluble
G-CSF receptor could be used in a clinical application for
acute myeloblastic leukemia patients in the future.