Abstract |
The possible involvement of the human T lymphotropic viruses type I and II (HTLV-I and -II) in lymphoproliferative disorders of mature T cells other than adult T cell leukemia/lymphoma ( ATLL) has been controversial. Most studies have focused primarily on the cutaneous T cell lymphomas. However, skin involvement is a frequent feature of T prolymphocytic leukemia (T-PLL) and antibodies against HTLV-I and -II have been reported in individuals with large granular lymphocytic ( LGL) leukemia. We examined 36 patients with T-PLL and 28 with LGL leukemia for evidence of HTLV-I and -II. Polymerase chain reaction (PCR) was performed on DNA from fresh peripheral blood mononuclear cells (PBMCs) and PBMCs after short-term culture (STC) using primers against all parts of the HTLV-I genome (LTR, gag, env, pol, tax/rex) and against HTLV-II pol and gag. Reverse transcriptase (RT) activity was measured on supernatants from STCs using a sensitive PCR-based technique. No HTLV-I or -II sequences were found by PCR nor RT activity detected in the 64 cases. Our findings do not provide evidence of HTLV-I or -II infection in T-PLL and LGL leukemia patients from an HTLV-I nonendemic area. Previous positive reports on these disorders may represent technical artefacts, detection of endogenous HTLV-like sequences or reflect patients from endemic areas and a variable etiology of T cell diseases.
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Authors | R Pawson, T F Schulz, E Matutes, D Catovsky |
Journal | Leukemia
(Leukemia)
Vol. 11
Issue 8
Pg. 1305-11
(Aug 1997)
ISSN: 0887-6924 [Print] England |
PMID | 9264385
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Review)
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Chemical References |
- RNA, Viral
- RNA-Directed DNA Polymerase
|
Topics |
- Human T-lymphotropic virus 1
- Human T-lymphotropic virus 2
- Humans
- Leukemia, Lymphoid
(virology)
- Leukemia, Prolymphocytic
(virology)
- Lymphoproliferative Disorders
(virology)
- Polymerase Chain Reaction
- RNA, Viral
(analysis)
- RNA-Directed DNA Polymerase
(analysis)
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