Abstract |
Gonadoblastomas are composed of nests of neoplastic germ cells and sex cord derivatives surrounded by ovarian-type stroma. These tumors are found almost exclusively in persons with gonadal dysgenesis associated with a Y chromosome or Y chromosome fragment, and accordingly, the Y chromosome has been implicated in gonadoblastoma oncogenesis. To evaluate this association, we used two-color fluorescence in situ hybridization with chromosome-specific probes to determine the distribution of the X and Y chromosomes in the tumor nests and surrounding stromal cells in paraffin tissue sections of three gonadoblastomas in two patients with gonadal dysgenesis and 45,X/46,XY mosaicism. Statistical analysis of the data from the fluorescence in situ hybridization demonstrated that in all three gonadoblastomas, the proportion of nuclei with a Y chromosome signal was significantly higher in the tumor cells than in the nontumoral cells of the surrounding stroma (P<.001). These results suggest that Y chromosome material participates in gonadoblastoma tumorigenesis.
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Authors | J C Iezzoni, C Von Kap-Herr, W L Golden, M J Gaffey |
Journal | American journal of clinical pathology
(Am J Clin Pathol)
Vol. 108
Issue 2
Pg. 197-201
(Aug 1997)
ISSN: 0002-9173 [Print] England |
PMID | 9260761
(Publication Type: Case Reports, Journal Article)
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Topics |
- Adolescent
- Adult
- Child
- Female
- Gonadal Dysgenesis, 46,XY
(genetics, pathology)
- Gonadoblastoma
(genetics, pathology)
- Humans
- In Situ Hybridization, Fluorescence
(methods)
- Male
- Middle Aged
- Mosaicism
(genetics)
- Ovarian Neoplasms
(genetics, pathology)
- Ovary
(chemistry)
- Skin
(chemistry)
- X Chromosome
(genetics, pathology)
- Y Chromosome
(genetics, pathology)
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