Abstract |
The effects of dietary bioantimutagens (compounds which have been shown to inhibit mutagenesis via interaction with DNA repair processes) on spontaneous and heterocyclic amine (HCA)-induced micronucleus (MN) frequencies were studied in metabolically competent human hepatoma (Hep-G2) cells. All the compounds tested ( coumarin, vanillin, caffeine, tannic acid and cinnamaldehyde) caused a moderate increase of MN numbers in Hep-G2 cells at high concentrations (500 microg/ml); only tannic acid was also active at lower dose levels. In combination experiments with the HCA 2-amino-3-methylimidazo-[3,4-f] quinoline (IQ), post-treatment of the cells with bioantimutagens resulted in a pronounced (75-90%) decrease in MN. The most drastic effects were seen with vanillin, coumarin and caffeine which were active at concentrations < or = 5 microg/ml. Further experiments indicated that these compounds also attenuate the mutagenic effects of other HCAs ( PhIP, MeIQ, MeIQx, Trp-P-1).
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Authors | R Sanyal, F Darroudi, W Parzefall, M Nagao, S Knasmüller |
Journal | Mutagenesis
(Mutagenesis)
Vol. 12
Issue 4
Pg. 297-303
(Jul 1997)
ISSN: 0267-8357 [Print] England |
PMID | 9237777
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amines
- Antimutagenic Agents
- Benzaldehydes
- Carbolines
- Coumarins
- Heterocyclic Compounds
- Hydrolyzable Tannins
- Imidazoles
- Mutagens
- Quinolines
- 2-amino-3-methylimidazo(4,5-f)quinoline
- Caffeine
- Acrolein
- 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine
- coumarin
- vanillin
- 2-amino-3,4-dimethylimidazo(4,5-f)quinoline
- 3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole
- cinnamaldehyde
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Topics |
- Acrolein
(analogs & derivatives, pharmacology)
- Amines
(toxicity)
- Antimutagenic Agents
(pharmacology)
- Benzaldehydes
(pharmacology)
- Caffeine
(pharmacology)
- Carbolines
(toxicity)
- Carcinoma, Hepatocellular
(drug therapy, genetics)
- Coumarins
(pharmacology)
- DNA Repair
(drug effects)
- Heterocyclic Compounds
(toxicity)
- Humans
- Hydrolyzable Tannins
(pharmacology)
- Imidazoles
(toxicity)
- Micronucleus Tests
- Mutagens
(toxicity)
- Quinolines
(toxicity)
- Tumor Cells, Cultured
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