Abstract |
Rapid and precise virus detection procedures are an important component of any epizootiological study. An automated one tube reverse transcriptase and nested primer polymerase chain reaction (RT-PCR) followed by nucleotide sequencing of the cDNA product, was used for the rapid detection and identification of louping ill (LI) virus in field caught Ixodes ricinus and compared with a classical isolation method i.e. infectivity in cell culture. The results establish the genetic identity of LI virus on the Lochindorb Estate. There was a high correlation between the results obtained by RT-PCR and infectivity assays. RT-PCR and sequencing proved to be a rapid and accurate system for identifying LI virus in field specimens. Development of this system should improve the capacity to undertake detailed epizootiological studies of LI virus.
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Authors | M W Gaunt, L D Jones, K Laurenson, P J Hudson, H W Reid, E A Gould |
Journal | Archives of virology
(Arch Virol)
Vol. 142
Issue 6
Pg. 1181-91
( 1997)
ISSN: 0304-8608 [Print] Austria |
PMID | 9229007
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Viral
- DNA, Viral
- Viral Envelope Proteins
- NEG envelope glycoprotein, Negishi virus
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Topics |
- Animals
- Antigens, Viral
- Base Sequence
- Cricetinae
- DNA, Viral
- Encephalitis Viruses, Tick-Borne
(classification, genetics, isolation & purification)
- Fluorescent Antibody Technique, Indirect
- Genetic Variation
- Ixodes
(virology)
- Mesocricetus
- Molecular Sequence Data
- Polymerase Chain Reaction
- Viral Envelope Proteins
(genetics)
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