Abstract |
Viral susceptibility of a newly established cell line, named KMP, derived from the peritoneal cavity of BALB/C mouse is described. The cells were originally cloned from the in vitro culture of ascites of the mouse injected with Ehrlich ascitic tumor cells in advance. The electrophoretic pattern of cellular DNAs, extracted from KMP, normal BALB/C mouse spleen, and Ehrlich tumor cells respectively were compared after triple digestions with restriction endonucleases. This cell line was proved to be of mouse origin, but not the sub-line of Ehrlich tumor cells. The strains of Coxsackie virus B group, swine enterovirus, influenza virus, encephalomyocarditis virus, vesicular stomatitis virus, and Aujeszky's disease virus were able to multiply well in the cell line with considerably high infectious titers in showing clear CPE and circular plaques.
|
Authors | K Kadoi, H Kamata, M Morita |
Journal | The new microbiologica
(New Microbiol)
Vol. 20
Issue 2
Pg. 149-54
(Apr 1997)
ISSN: 1121-7138 [Print] Italy |
PMID | 9208425
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
|
Topics |
- Animals
- Carcinoma, Ehrlich Tumor
(genetics)
- Cell Culture Techniques
(methods)
- Cell Line
(virology)
- Clone Cells
(virology)
- DNA
(analysis)
- Disease Susceptibility
(virology)
- Mice
- Mice, Inbred BALB C
(virology)
- Peritoneal Cavity
(cytology, virology)
- Spleen
(cytology)
- Viral Plaque Assay
- Virus Diseases
(virology)
|