Cloning and molecular analysis of the Aspergillus terreus arg1 gene coding for an ornithine carbamoyltransferase.

Abstract

An Aspergillus terreus gene (arg1) encoding ornithine carbamoyltransferase (OCTase) has been cloned and sequenced. The deduced amino acid sequence contains a signal peptide suggestive of a mitochondrial location for the A. terreus enzyme. Alignment of the A. terreus OCTase sequence with other OCTases revealed the presence of conserved regions. Northern analysis indicates that arg1 expression is regulated at the level of transcription and that transcription of the arg1 gene is not markedly affected by arginine sufficiency. However, histidine starvation effected by 3-amino-1,2,4-triazole increased transcription of the arg1 gene, indicating cross-pathway regulation of OCTase synthesis in A. terreus.

• Authors: L Ventura, J A Pérez-González, D Ramón
• Journal: FEMS microbiology letters (FEMS Microbiol Lett) Vol. 149 Issue 2 Pg. 207-12 (Apr 15 1997) ISSN: 0378-1097 [Print] England
• PMID: 9141661 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amino Acids
• Ornithine Carbamoyltransferase

Topics

• Amino Acids (metabolism)
• Aspergillus (enzymology, genetics)
• Base Sequence
• Cloning, Molecular
• Gene Expression Regulation, Enzymologic (physiology)
• Gene Expression Regulation, Fungal (physiology)
• Genes, Fungal (genetics)
• Molecular Sequence Data
• Ornithine Carbamoyltransferase (genetics)
• Sequence Analysis, DNA
• Sequence Homology, Amino Acid