CeReS-18, a novel cell surface sialoglycopeptide, induces cell cycle arrest and apoptosis in a calcium-sensitive manner.

Very few growth inhibitors have been identified which can inhibit the proliferation of a broad spectrum of human breast cancer cell lines. CeReS-18, a novel cell surface sialoglycopeptide growth inhibitor, can reversibly inhibit the proliferation of both estrogen receptor positive (MCF-7) and negative (BT-20) human breast cancer cell lines. In addition, at concentrations above those required for the reversible inhibition of cell proliferation, CeReS-18 can also induce cell death in MCF-7 cells. Changes in nuclear and cytoplasmic morphology, characteristic of apoptosis, were detected in MCF-7 cells treated with a cytotoxic concentration of CeReS-18, and internucleosomal DNA cleavage was also observed. The sensitivity of MCF-7 and BT-20 cells to the biological properties of CeReS-18 could be influenced by altering the calcium concentration in the extracellular growth medium, such that when the calcium concentration in the environment was decreased, and increased sensitivity to CeReS-18-induced growth inhibition and cytotoxicity were observed. The addition of the calcium chelating agent EGTA to MCF-7 cells, cultured in a normal calcium environment, could mimic the increased sensitivity to the biological effects of CeReS-18 observed under reduced calcium conditions.
AuthorsN A Betz, H K Fattaey, B A Westhoff, A Q Paulsen, T C Johnson
JournalBreast cancer research and treatment (Breast Cancer Res Treat) Vol. 42 Issue 2 Pg. 137-48 (Jan 1997) ISSN: 0167-6806 [Print] NETHERLANDS
PMID9138603 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Antineoplastic Agents
  • Receptors, Estrogen
  • Sialoglycoproteins
  • Calcium
  • Antineoplastic Agents (pharmacology)
  • Apoptosis (drug effects)
  • Breast Neoplasms (drug therapy, pathology, ultrastructure)
  • Calcium (metabolism)
  • Cell Cycle (drug effects)
  • Cell Division (drug effects)
  • Drug Screening Assays, Antitumor
  • Extracellular Space (metabolism)
  • Humans
  • Receptors, Estrogen (physiology)
  • Sialoglycoproteins (pharmacology)
  • Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: