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CeReS-18, a novel cell surface sialoglycopeptide, induces cell cycle arrest and apoptosis in a calcium-sensitive manner.

Abstract
Very few growth inhibitors have been identified which can inhibit the proliferation of a broad spectrum of human breast cancer cell lines. CeReS-18, a novel cell surface sialoglycopeptide growth inhibitor, can reversibly inhibit the proliferation of both estrogen receptor positive (MCF-7) and negative (BT-20) human breast cancer cell lines. In addition, at concentrations above those required for the reversible inhibition of cell proliferation, CeReS-18 can also induce cell death in MCF-7 cells. Changes in nuclear and cytoplasmic morphology, characteristic of apoptosis, were detected in MCF-7 cells treated with a cytotoxic concentration of CeReS-18, and internucleosomal DNA cleavage was also observed. The sensitivity of MCF-7 and BT-20 cells to the biological properties of CeReS-18 could be influenced by altering the calcium concentration in the extracellular growth medium, such that when the calcium concentration in the environment was decreased, and increased sensitivity to CeReS-18-induced growth inhibition and cytotoxicity were observed. The addition of the calcium chelating agent EGTA to MCF-7 cells, cultured in a normal calcium environment, could mimic the increased sensitivity to the biological effects of CeReS-18 observed under reduced calcium conditions.
AuthorsN A Betz, H K Fattaey, B A Westhoff, A Q Paulsen, T C Johnson
JournalBreast cancer research and treatment (Breast Cancer Res Treat) Vol. 42 Issue 2 Pg. 137-48 (Jan 1997) ISSN: 0167-6806 [Print] NETHERLANDS
PMID9138603 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Antineoplastic Agents
  • Receptors, Estrogen
  • Sialoglycoproteins
  • Calcium
Topics
  • Antineoplastic Agents (pharmacology)
  • Apoptosis (drug effects)
  • Breast Neoplasms (drug therapy, pathology, ultrastructure)
  • Calcium (metabolism)
  • Cell Cycle (drug effects)
  • Cell Division (drug effects)
  • Drug Screening Assays, Antitumor
  • Extracellular Space (metabolism)
  • Humans
  • Receptors, Estrogen (physiology)
  • Sialoglycoproteins (pharmacology)
  • Tumor Cells, Cultured

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