This paper presents the results of researched influence of systematic physical effort together with a
calorie restricted diet on fourteen-year-old obese boys. We evaluated their physical efficiency (VO2max), body consistency, blood
lipid profile the level of non-enzymatic glycosylation of
protein, insulinemia, and
insulin resistance measured by the binding of 125I-Insulin to erythrocyte receptors. For 21 days, subjects performed 30 minutes of physical effort on a bicycle ergometer with load of 1 W/kg of body mass. Simultaneously, they were subjected to a
calorie restricted diet at an average level of 1300 kcal. Before administering of the
therapy and following its completion, the maximal amount of
oxygen intake and body consistency was determined. Following that, the boy subjects performed an effort on the bicycle ergometer having an intensity of 70% VO2max and, simultaneously, capillary and venous blood was drawn for testing. In the capillary blood samples indices of acid-base balance, the
lactate level, and
glucose level were determined. In the venous blood samples levels of
lipids, immunoreactive
insulin,
C-peptide and
fructosamine were determined. Binding of 125I-Insulin was determined according to the method described by Gambhir and modified by us. Obtained results show that the administered
therapy contributed to an increased physical efficiency, a decrease in the body's fatty mass, a reduction by 20% of insulinemia (p < 0.01) and
insulin resistance as measured by the amount of 125I-Insulin binding.