Mouse strain and source of L-tryptophan affects hepatic nuclear tryptophan binding.

Abstract

This study describes that the affinity for specific L-tryptophan binding to hepatic nuclei in vitro is markedly decreased in NZBWF1 mice in comparison to that in Swiss mice. Also, the hepatic nuclei of NZBWF1 mice have a significantly decreased binding response in vitro to Showa Denko L-tryptophan (implicated in the eosinophilia-myalgia syndrome) or to its contaminants, 1,1'-ethylidenebis(tryptophan) or 3-phenylamino-L-alanine, when each is added to control, non-implicated L-tryptophan compared with hepatic nuclei of Swiss mice. Enhanced hepatic protein synthesis induced by tube-feeding control L-tryptophan is much less in NZBWF1 mice than in Swiss mice. Tube-feeding of Showa Denko L-tryptophan induced less stimulation of hepatic protein synthesis than did control L-tryptophan in Swiss mice but essentially none in NZBWF1 mice. NZBWF1 mice have a genetically altered response to L-tryptophan which may prove to be useful is studying the role of L-tryptophan in health and in disease.

• Authors: H Sidransky, E Verney
• Journal: Toxicology (Toxicology) Vol. 118 Issue 1 Pg. 37-47 (Mar 14 1997) ISSN: 0300-483X [Print] Ireland
• PMID: 9074652 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Selenium Compounds
• 1,1'-ethylidene bis(tryptophan)
• 3-(phenylamino)alanine
• Tryptophan
• Sodium Selenite
• Selenic Acid
• Alanine

Topics

• Alanine (analogs & derivatives, pharmacology)
• Animals
• Binding, Competitive
• Cell Nucleus (metabolism)
• Liver (metabolism, ultrastructure)
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Inbred DBA
• Mice, Inbred NZB
• Protein Biosynthesis
• Selenic Acid
• Selenium Compounds (pharmacology)
• Sodium Selenite (pharmacology)
• Tryptophan (analogs & derivatives, chemistry, metabolism)