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Z-321, a prolyl endopeptidase inhibitor, augments the potentiation of synaptic transmission in rat hippocampal slices.

Abstract
The present study investigated the effects of arginine-vasopressin (AVP) and (1-[3-(2-indanylacetyl)-L-thioprolyl] pyrrolidine (Z-321), an inhibitor of prolyl endopeptidase (PEP; (EC 3.4.21.26)) which degrades AVP in vitro, on the short-lasting potentiation of the field excitatory postsynaptic potentials (EPSP) coupled with a weak tetanus. The EPSP, after the electrical stimulation of the Schaffer collateral/commissural pathway, were recorded in the CA1 region of rat hippocampal slices. AVP at 10(-8) M and Z-321 at 10(-4) M augmented the potentiation induced by the weak tetanus; the magnitude of the post-tetanic potentiation of the EPSP was enhanced and the potentiation lasted for 60 min. In contrast, the racemic D-thioprolyl compound of Z-321, which virtually lacks any inhibitory effects on PEP, failed to affect the potentiation at 10(-4) M. The facilitatory effect of Z-321 was reversed by the application of [d(CH2)5,Tyr(Me)2]AVP (10(-8) M), an antagonist of the AVP V1 receptors, indicating that the effect of Z-321 was mediated through the V1 receptors. These findings suggest that Z-321 augmented the potentiation due to its inhibitory influence on the AVP degradation by PEP.
AuthorsN Miura, S Shibata, S Watanabe
JournalBehavioural brain research (Behav Brain Res) Vol. 83 Issue 1-2 Pg. 213-6 (Feb 1997) ISSN: 0166-4328 [Print] NETHERLANDS
PMID9062687 (Publication Type: Journal Article)
Chemical References
  • 1-(3-(2-indanylacetyl)-L-thioprolyl)pyrrolidine
  • Pyrrolidines
  • Serine Proteinase Inhibitors
  • Arginine Vasopressin
  • Serine Endopeptidases
  • prolyl oligopeptidase
Topics
  • Animals
  • Arginine Vasopressin (pharmacology)
  • Electric Stimulation
  • Evoked Potentials (drug effects)
  • Hippocampus (drug effects)
  • In Vitro Techniques
  • Male
  • Pyrrolidines (pharmacology)
  • Rats
  • Rats, Wistar
  • Serine Endopeptidases (metabolism)
  • Serine Proteinase Inhibitors (pharmacology)
  • Stimulation, Chemical
  • Synaptic Transmission (drug effects)

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