Abstract | BACKGROUND: RESULTS: . The crystal structure of the enzyme has been solved and refined at 2.5 resolution using data recorded at both 123K and 273K. The structure has two domains, the larger of which belongs to the alpha/beta class of proteins and contains the active site. The enzyme active site in the crystals contains several hitherto undescribed features. The active-site cysteine residue, Cys91, is found as the sulfate derivative of the aldehyde species, oxo- alanine. The sulfate is bound to a previously undetected metal ion, which we have identified as calcium. The structure of a vanadate-inhibited form of the enzyme has also been solved, and this structure shows that vanadate has replaced sulfate in the active site and that the vanadate is covalently linked to the protein. Preliminary data is presented for crystals soaked in the monosaccharide N- acetylgalactosamine, the structure of which forms a product complex of the enzyme. CONCLUSIONS:
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Authors | C S Bond, P R Clements, S J Ashby, C A Collyer, S J Harrop, J J Hopwood, J M Guss |
Journal | Structure (London, England : 1993)
(Structure)
Vol. 5
Issue 2
Pg. 277-89
(Feb 15 1997)
ISSN: 0969-2126 [Print] United States |
PMID | 9032078
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Enzyme Inhibitors
- Recombinant Proteins
- Vanadates
- Alkaline Phosphatase
- Chondro-4-Sulfatase
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Topics |
- Alkaline Phosphatase
(chemistry)
- Amino Acid Sequence
- Animals
- Binding Sites
- CHO Cells
- Chondro-4-Sulfatase
(antagonists & inhibitors, chemistry, deficiency, genetics)
- Consensus Sequence
- Cricetinae
- Crystallography, X-Ray
- Enzyme Inhibitors
(metabolism, pharmacology)
- Glycosylation
- Humans
- Lysosomes
(enzymology)
- Models, Molecular
- Molecular Sequence Data
- Mucopolysaccharidosis VI
(enzymology, genetics)
- Multigene Family
- Point Mutation
- Protein Conformation
- Recombinant Proteins
(antagonists & inhibitors, chemistry, metabolism)
- Sequence Alignment
- Sequence Homology, Amino Acid
- Vanadates
(metabolism, pharmacology)
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