Antiallergic activity of topical lodoxamide on in vivo and in vitro models.

Abstract

Lodoxamide is an antiallergic drug acting as a mast-cell stabilizer, which is effective in the treatment of allergic conjunctivitis. The study aimed to evaluate the effect of lodoxamide eye-drops on the inflammatory early-phase reaction (EPR) changes induced by allergen-specific conjunctival challenge (ASCC). This was a cross-over, double-blind, placebo-controlled, randomized study, including 10 outpatients suffering from allergic rhinoconjunctivitis due to Parietaria judaica. Patients received one drop of lodoxamide tromethamine 0.1% or placebo 30 min before each ASCC. Clinical evaluation and cytologic assessment were done at baseline and 30 min after each ASCC. Lodoxamide induced a reduction in total symptom score and hyperemia during the EPR (P < 0.05). Lacrimation, itching/burning, and eyelid swelling were only slightly (nonsignificantly) reduced. Lodoxamide induced a reduction in the total number of inflammatory cells and neutrophils during the EPR (P < 0.02). Eosinophil and lymphocyte number and ICAM-1 expression showed only a slight, not statistically significant decrease. Placebo did not affect the studied parameters. Lodoxamide reduced early clinical events and cellular changes after ASCC consistently with its activity as mast-cell stabilizer. Moreover, lodoxamide was able to downregulate in vitro ICAM-1 expression on the continuously cultured, differentiated conjunctival cell line WK. This was shown both in basal conditions (P < 0.05) and upon interferon-gamma stimulation (P < 0.03), although at high concentration.

• Authors: G Ciprandi, S Buscaglia, A Catrullo, F Paolieri, A M Riccio, N Fiorino, G W Canonica
• Journal: Allergy (Allergy) Vol. 51 Issue 12 Pg. 946-51 (Dec 1996) ISSN: 0105-4538 [Print] Denmark
• PMID: 9020426 (Publication Type: Clinical Trial, Comparative Study, Journal Article, Randomized Controlled Trial)

Chemical References

• Intercellular Adhesion Molecule-1
• Oxamic Acid
• lodoxamide ethyl

Topics

• Adolescent
• Adult
• Cell Line
• Conjunctiva (cytology, drug effects, metabolism)
• Conjunctivitis, Allergic (chemically induced, drug therapy)
• Cross-Over Studies
• Double-Blind Method
• Epithelium (drug effects, metabolism)
• Female
• Humans
• Intercellular Adhesion Molecule-1 (biosynthesis)
• Male
• Middle Aged
• Oxamic Acid (analogs & derivatives, pharmacology, therapeutic use)