Abstract | OBJECTIVE: METHODS AND DESIGN: OUTCOME MEASURES: Middle ear fluid volume and albumin content were determined as measures of vascular extravasation. Histological sections of the middle ear mucosa were used to quantify the degree of leukocyte exudation. Data were analyzed by 1- or 2-way analysis of variance with the significance level set at P < .05. RESULTS:
Lipopolysaccharide exposure caused a significant increase in the mean +/- SEM middle ear fluid volume from 29.9 +/- 0.99 to 45.8 +/- 1.4 microL between the 2- and 6-hour samplings. Lipopolysaccharide exposure caused a significant increase in the albumin content of middle ear fluid from 70.1 +/- 19.2 to 271.0 +/- 93.1 micrograms between the 2- and 6-hour samplings. Both increases were significant compared with controls. Lipopolysaccharide also caused a significant increase in leukocytes localized to the middle ear mucosa. Pretreatment of animals with dexamethasone for either 2 or 24 hours inhibited the lipopolysaccharide-induced changes. There were no differences between 2- and 24-hour pretreatment with dexamethasone. CONCLUSIONS:
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Authors | H C Baggett, J Prazma, A S Rose, A P Lane, H C Pillsbury 3rd |
Journal | Archives of otolaryngology--head & neck surgery
(Arch Otolaryngol Head Neck Surg)
Vol. 123
Issue 1
Pg. 41-6
(Jan 1997)
ISSN: 0886-4470 [Print] United States |
PMID | 9006502
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Albumins
- Glucocorticoids
- Lipopolysaccharides
- Dexamethasone
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Topics |
- Albumins
(metabolism)
- Animals
- Dexamethasone
(pharmacology)
- Evaluation Studies as Topic
- Exudates and Transudates
(cytology, metabolism)
- Glucocorticoids
(pharmacology)
- Leukocytes
- Lipopolysaccharides
(pharmacology)
- Otitis Media with Effusion
(drug therapy, etiology, pathology, physiopathology)
- Rats
- Rats, Sprague-Dawley
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