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Topical cyclosporine inhibits mast cell-mediated conjunctivitis.

AbstractPURPOSE:
Allergic conjunctivitis is a common condition caused by a mast cell-mediated hypersensitivity reaction to immunoglobulin E-bound allergens. The purpose of this study was to investigate the effect of topical cyclosporine A on the development of mast cell-mediated conjunctivitis in mice.
METHODS:
Allergic conjunctivitis was induced in C57BL/6 mice by topical applications of compound 48/80, a mast cell degranulating agent. In two separate experiments, mice were treated with topical cyclosporine A (0.05%, 0.2%, or 0.4%), prednisolone acetate 1%, or phosphate-buffered saline. Twenty-four hours after compound 48/80 instillation, the number of neutrophils, eosinophils, lymphocytes, macrophages, and the number of preserved goblet cells and undegranulated mast cells in the conjunctiva were counted by a masked observer.
RESULTS:
In both experiments, treatment with all three doses of cyclosporine A resulted in a statistically significant reduction in the number of infiltrating neutrophils and eosinophils compared to saline-treated controls. There was no significant difference in the treatment effect of cyclosporine and prednisolone acetate. In addition, there was increased preservation of goblet cells in the cyclosporine A-treated animals. Immunohistochemical staining showed a reduction in infiltrating lymphocytes and a smaller reduction in infiltrating macrophages in animals treated with cyclosporine compared to saline-treated controls.
CONCLUSIONS:
Topical cyclosporine A was effective in inhibiting the development of mast cell-mediated allergic conjunctivitis in mice. This study suggests that topical cyclosporine A may be effective in treating allergic conjunctivitis in humans.
AuthorsS M Whitcup, C C Chan, D A Luyo, P Bo, Q Li
JournalInvestigative ophthalmology & visual science (Invest Ophthalmol Vis Sci) Vol. 37 Issue 13 Pg. 2686-93 (Dec 1996) ISSN: 0146-0404 [Print] United States
PMID8977483 (Publication Type: Journal Article)
Chemical References
  • Anti-Inflammatory Agents
  • Immunosuppressive Agents
  • Ophthalmic Solutions
  • p-Methoxy-N-methylphenethylamine
  • Cyclosporine
  • prednisolone acetate
  • Prednisolone
Topics
  • Administration, Topical
  • Animals
  • Anti-Inflammatory Agents (pharmacology)
  • Conjunctivitis, Allergic (chemically induced, pathology, prevention & control)
  • Cyclosporine (administration & dosage, pharmacology)
  • Female
  • Immunosuppressive Agents (administration & dosage, pharmacology)
  • Leukocyte Count
  • Lymphocytes (drug effects, pathology)
  • Macrophages (drug effects, pathology)
  • Mast Cells (drug effects)
  • Mice
  • Mice, Inbred C57BL
  • Neutrophils (drug effects, pathology)
  • Ophthalmic Solutions
  • Prednisolone (analogs & derivatives, pharmacology)
  • p-Methoxy-N-methylphenethylamine (toxicity)

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