Abstract | PURPOSE: METHODS:
Allergic conjunctivitis was induced in C57BL/6 mice by topical applications of compound 48/80, a mast cell degranulating agent. In two separate experiments, mice were treated with topical cyclosporine A (0.05%, 0.2%, or 0.4%), prednisolone acetate 1%, or phosphate-buffered saline. Twenty-four hours after compound 48/80 instillation, the number of neutrophils, eosinophils, lymphocytes, macrophages, and the number of preserved goblet cells and undegranulated mast cells in the conjunctiva were counted by a masked observer. RESULTS: In both experiments, treatment with all three doses of cyclosporine A resulted in a statistically significant reduction in the number of infiltrating neutrophils and eosinophils compared to saline-treated controls. There was no significant difference in the treatment effect of cyclosporine and prednisolone acetate. In addition, there was increased preservation of goblet cells in the cyclosporine A-treated animals. Immunohistochemical staining showed a reduction in infiltrating lymphocytes and a smaller reduction in infiltrating macrophages in animals treated with cyclosporine compared to saline-treated controls. CONCLUSIONS:
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Authors | S M Whitcup, C C Chan, D A Luyo, P Bo, Q Li |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 37
Issue 13
Pg. 2686-93
(Dec 1996)
ISSN: 0146-0404 [Print] United States |
PMID | 8977483
(Publication Type: Journal Article)
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Chemical References |
- Anti-Inflammatory Agents
- Immunosuppressive Agents
- Ophthalmic Solutions
- p-Methoxy-N-methylphenethylamine
- Cyclosporine
- prednisolone acetate
- Prednisolone
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Topics |
- Administration, Topical
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- Conjunctivitis, Allergic
(chemically induced, pathology, prevention & control)
- Cyclosporine
(administration & dosage, pharmacology)
- Female
- Immunosuppressive Agents
(administration & dosage, pharmacology)
- Leukocyte Count
- Lymphocytes
(drug effects, pathology)
- Macrophages
(drug effects, pathology)
- Mast Cells
(drug effects)
- Mice
- Mice, Inbred C57BL
- Neutrophils
(drug effects, pathology)
- Ophthalmic Solutions
- Prednisolone
(analogs & derivatives, pharmacology)
- p-Methoxy-N-methylphenethylamine
(toxicity)
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