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Selective loss of neurofilament proteins after exposure of differentiated human IMR-32 neuroblastoma cells to oxidative stress.

Abstract
Millimolar concentrations of ascorbate in the presence of iron can cause neuronal cell death. This study shows that the human neuronal cell line IMR-32 is sensitive to ascorbate and that cytotoxicity can be blocked by the antioxidant enzymes Cu/Zn-superoxide dismutase and catalase. There was a selective loss of neurofilament proteins after exposure to 5 or 10 mM ascorbate, as assessed by immunostaining and by Western blotting. Loss of actin or tubulin was not seen, suggesting that loss of neurofilaments is a sensitive and selective marker for free radical damage in these cells.
AuthorsM R Cookson, N M Thatcher, P G Ince, P J Shaw
JournalBrain research (Brain Res) Vol. 738 Issue 1 Pg. 162-6 (Oct 28 1996) ISSN: 0006-8993 [Print] Netherlands
PMID8949942 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Neurofilament Proteins
  • Catalase
  • Superoxide Dismutase
  • Ascorbic Acid
Topics
  • Ascorbic Acid (pharmacology)
  • Blotting, Western
  • Catalase (pharmacology)
  • Cell Death
  • Cell Differentiation
  • Dose-Response Relationship, Drug
  • Humans
  • Immunologic Techniques
  • Neurofilament Proteins (metabolism)
  • Oxidative Stress (physiology)
  • Staining and Labeling
  • Superoxide Dismutase (pharmacology)
  • Time Factors
  • Tumor Cells, Cultured (drug effects, pathology, ultrastructure)

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