Abstract |
Millimolar concentrations of ascorbate in the presence of iron can cause neuronal cell death. This study shows that the human neuronal cell line IMR-32 is sensitive to ascorbate and that cytotoxicity can be blocked by the antioxidant enzymes Cu/Zn-superoxide dismutase and catalase. There was a selective loss of neurofilament proteins after exposure to 5 or 10 mM ascorbate, as assessed by immunostaining and by Western blotting. Loss of actin or tubulin was not seen, suggesting that loss of neurofilaments is a sensitive and selective marker for free radical damage in these cells.
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Authors | M R Cookson, N M Thatcher, P G Ince, P J Shaw |
Journal | Brain research
(Brain Res)
Vol. 738
Issue 1
Pg. 162-6
(Oct 28 1996)
ISSN: 0006-8993 [Print] Netherlands |
PMID | 8949942
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Neurofilament Proteins
- Catalase
- Superoxide Dismutase
- Ascorbic Acid
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Topics |
- Ascorbic Acid
(pharmacology)
- Blotting, Western
- Catalase
(pharmacology)
- Cell Death
- Cell Differentiation
- Dose-Response Relationship, Drug
- Humans
- Immunologic Techniques
- Neurofilament Proteins
(metabolism)
- Oxidative Stress
(physiology)
- Staining and Labeling
- Superoxide Dismutase
(pharmacology)
- Time Factors
- Tumor Cells, Cultured
(drug effects, pathology, ultrastructure)
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