Abstract |
A no-carrier-added synthesis of 9-[(3-[18F]-fluoro-1-hydroxy-2-propoxy)methyl]- guanine ([18F]-FHPG) is reported. The 9-[(1,3-dihydroxy-2-propoxy)methyl) guanine ( DHPG) was converted to 9-[N2,O-bis(methoxytrityl)-3-(tosyl)-2-propoxy-methyl] guanine by treatment with methoxytrityl chloride followed by tosylation. The tosylate was reacted with [18F]-KF in the presence of kryptofix 2.2.2. to produce the 3-fluoro-N2-O-bis-(methoxytrityl) derivative. Removal of the methoxytrityl protecting groups by acid hydrolysis produced [18F]-FHPG. The labeled product was purified by HPLC on a reverse-phase C18 column, and eluted in 9 min with a mobile phase of 5% acetonitrile in water. The radiochemical yield was 7-17%, with an average of 10% in 10 runs (corrected for decay to EOB). The radiochemical purity was > 99%, and specific activities with an average of 526 mCi/mumol were obtained. The synthesis time was 70-80 min, including HPLC purification and determination of radiochemical purity and specific activity.
|
Authors | M M Alauddin, P S Conti, S M Mazza, F M Hamzeh, J R Lever |
Journal | Nuclear medicine and biology
(Nucl Med Biol)
Vol. 23
Issue 6
Pg. 787-92
(Aug 1996)
ISSN: 0969-8051 [Print] United States |
PMID | 8940722
(Publication Type: Journal Article)
|
Chemical References |
- 9-((3-fluoro-1-hydroxy-2-propoxy)methyl)guanine
- Antiviral Agents
- Fluorine Radioisotopes
- Ganciclovir
|
Topics |
- Antiviral Agents
(chemistry, pharmacokinetics)
- Chromatography, High Pressure Liquid
- Fluorine Radioisotopes
(chemistry)
- Ganciclovir
(analogs & derivatives, chemical synthesis, pharmacokinetics)
- Genetic Therapy
- Isotope Labeling
- Radiochemistry
(methods)
- Tomography, Emission-Computed
|