Interaction of human lung surfactant proteins A and D with mite (Dermatophagoides pteronyssinus) allergens.

Abstract

Human lung surfactant proteins A (SP-A) and D (SP-D) are both collagenous C-type lectins which appear to mediate antimicrobial activity by binding to carbohydrates on micro-organisms and to receptors on phagocytic cells. Purified native SP-A and SP-D, isolated from human bronchoalveolar lavage fluid, were found to bind to whole mite extracts (Dermatophagoides pteronyssinus) and the purified allergen Der p I, in a carbohydrate-specific and calcium-dependent manner. Binding was inhibited by ethylenediamine tetra-acetic acid (EDTA) as well as by maltose in the case of SP-D, or mannose in the case of SP-A. A recombinant polypeptide, which trimerized to form the neck region and carbohydrate recognition domains of SP-D, also inhibited the binding of native SP-D to the whole mite extract and Der p I. Both SP-A and SP-D did not bind to deglycosylated whole mite extracts or to recombinant Der p proteins, which lacked carbohydrate residues. These results suggest that the ability of surfactant proteins to bind certain allergens is mediated through their carbohydrate-recognition domains (CRDs) interacting with carbohydrate residues on the allergens. Moreover, SP-A and SP-D were found to inhibit allergen-specific IgE binding to the mite extracts either via steric hindrance or competitive binding. It is therefore possible that SP-A and SP-D may be involved in the modulation of allergen sensitization and/or the development of allergic reactions.

• Authors: J Y Wang, U Kishore, B L Lim, P Strong, K B Reid
• Journal: Clinical and experimental immunology (Clin Exp Immunol) Vol. 106 Issue 2 Pg. 367-73 (Nov 1996) ISSN: 0009-9104 [Print] England
• PMID: 8918587 (Publication Type: Journal Article)

Chemical References

• Allergens
• Antigens
• Antigens, Dermatophagoides
• Carrier Proteins
• Glycoproteins
• Proteolipids
• Pulmonary Surfactant-Associated Protein A
• Pulmonary Surfactant-Associated Protein D
• Pulmonary Surfactant-Associated Proteins
• Pulmonary Surfactants
• Recombinant Proteins
• Maltose
• Edetic Acid
• Mannose
• Calcium

Topics

• Allergens (metabolism)
• Animals
• Antigens (metabolism)
• Antigens, Dermatophagoides
• Binding, Competitive (drug effects)
• Bronchoalveolar Lavage Fluid (chemistry)
• Calcium (pharmacology)
• Carrier Proteins (metabolism)
• Edetic Acid (pharmacology)
• Glycoproteins (isolation & purification, metabolism)
• Humans
• Maltose (pharmacology)
• Mannose (pharmacology)
• Mites (metabolism)
• Proteolipids (isolation & purification, metabolism)
• Pulmonary Surfactant-Associated Protein A
• Pulmonary Surfactant-Associated Protein D
• Pulmonary Surfactant-Associated Proteins
• Pulmonary Surfactants (isolation & purification, metabolism)
• Recombinant Proteins