Treatment of intracellular Mycobacterium avium complex infection by free and liposome-encapsulated sparfloxacin.
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| Abstract |
Mycobacterium avium-M. intracellulare complex (MAC) is the most frequent cause of opportunistic bacterial infection in patients with AIDS. Previous studies have indicated that liposome-encapsulated aminoglycosides are highly effective in treating MAC infections in mice. We investigated whether the fluoroquinolone sparfloxacin is effective in treating MAC infection in the murine macrophage-like cell line J774. Sparfloxacin was encapsulated in the membrane phase of multilamellar liposomes composed of phosphatidylglycerol-phosphatidylcholine-cholesterol (1:1:1 molar ratio). MAC-infected macrophages were treated for either 24 h or 4 days with free or liposome-encapsulated sparfloxacin. Treatment with free or liposome-encapsulated sparfloxacin (6 micrograms/ml) for 24 h resulted in the reduction of the growth index to 25 and 30% of that of untreated controls, respectively. When cultures were treated for 4 days, free sparfloxacin reduced the growth index to 6% of that of the untreated control, while liposome-encapsulated sparfloxacin reduced it to 8% of that of the control.
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| Authors | N Düzgüneş, D Flasher, M V Reddy, J Luna-Herrera, P R Gangadharam |
| Journal | Antimicrobial agents and chemotherapy (Antimicrob Agents Chemother) Vol. 40 Issue 11 Pg. 2618-21 (Nov 1996) ISSN: 0066-4804 [Print] United States |
| PMID | 8913475 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.) |
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