Involvement of antipain-sensitive protease activity in the interferon-beta-induced UV-refractoriness of Cockayne syndrome fibroblasts.

Abstract

Fibroblast cells obtained from two siblings and a female patient with Cockayne syndrome (CS), when pretreated with human interferon (HuIFN)-beta prior to irradiation with UV light (254 nm wavelength), exhibited transiently induced fibrinolytic protease activity immediately after the irradiation in association with increased refractoriness to UV cell-killing. A protease inhibitor, antipain, inhibited the induction of protease activity in lysates of the CS fibroblasts from these 3 cases after the combination of HuIFN-beta pretreatment and UV irradiation, whereas elastatinal and 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) inhibited the activity less than antipain did. Antipain also suppressed the increase in UV-refractoriness of HuIFN-beta-pretreated CS fibroblasts, as revealed by culturing cells for 24 h in medium containing the inhibitor immediately after UV exposure and thereafter evaluating the ability of colony formation by the cells. Thus, an antipain-sensitive protease may be involved in the UV-refractoriness induced by HuIFN-beta in CS fibroblast strains.

• Authors: K Sugita, N Suzuki, H Niimi
• Journal: Mutation research (Mutat Res) Vol. 357 Issue 1-2 Pg. 177-81 (Oct 25 1996) ISSN: 0027-5107 [Print] Netherlands
• PMID: 8876692 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Protease Inhibitors
• Antipain
• Interferon-beta
• Endopeptidases

Topics

• Antipain (pharmacology)
• Cell Survival (radiation effects)
• Cockayne Syndrome (genetics)
• Endopeptidases (metabolism)
• Female
• Humans
• Interferon-beta (physiology)
• Male
• Pedigree
• Protease Inhibitors (pharmacology)
• Ultraviolet Rays