Abstract |
Inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice was observed in the methanol extract of Chlorella vulgaris, a green alga. The hexane soluble fraction obtained from the methanol extract exhibited marked inhibitory activity from which were isolated two delta(5,7)-sterols ( ergosterol and 7-dehydroporiferasterol), two delta(5,7,9(11))-sterols [7,9(11)- dehydroergosterol and 7,9(11)-bisdehydroporiferasterol], two 5 alpha, 8 alpha-epidioxy-delta(6)-sterols ( ergosterol peroxide and 7-dehydroporiferasterol peroxide), and a 7-oxo-delta(5)-sterol (7-oxocholesterol), among others. The delta 5'7-sterols, 5 alpha, 8 alpha-epidioxy-delta(6)-sterols and 7-oxo-delta 5-sterol inhibited TPA-induced inflammation in mice. The 50% inhibitory dose of these compounds for TPA-induced inflammation was 0.2-0.7 mg/ear. Furthermore, ergosterol peroxide markedly inhibited the tumor-promoting effect of TPA in 7,12-dimethylbenz[a] anthracene-initiated mice.
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Authors | K Yasukawa, T Akihisa, H Kanno, T Kaminaga, M Izumida, T Sakoh, T Tamura, M Takido |
Journal | Biological & pharmaceutical bulletin
(Biol Pharm Bull)
Vol. 19
Issue 4
Pg. 573-6
(Apr 1996)
ISSN: 0918-6158 [Print] Japan |
PMID | 8860961
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anticarcinogenic Agents
- Carcinogens
- Cell Extracts
- Sterols
- 9,10-Dimethyl-1,2-benzanthracene
- Tetradecanoylphorbol Acetate
- ergosterol-5,8-peroxide
- Ergosterol
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Topics |
- 9,10-Dimethyl-1,2-benzanthracene
(pharmacology)
- Animals
- Anticarcinogenic Agents
(pharmacology)
- Carcinogens
(antagonists & inhibitors, pharmacology)
- Cell Extracts
(chemistry, pharmacology)
- Chlorella
(chemistry)
- Chromatography
- Ergosterol
(analogs & derivatives, pharmacology, therapeutic use)
- Female
- Inflammation
(chemically induced, prevention & control)
- Mice
- Mice, Inbred Strains
- Molecular Structure
- Skin
(drug effects)
- Skin Diseases
(chemically induced, prevention & control)
- Sterols
(chemistry, pharmacology)
- Tetradecanoylphorbol Acetate
(antagonists & inhibitors, pharmacology)
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