Inhibitory effects of sterols isolated from Chlorella vulgaris on 12-0-tetradecanoylphorbol-13-acetate-induced inflammation and tumor promotion in mouse skin.

Abstract	Inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice was observed in the methanol extract of Chlorella vulgaris, a green alga. The hexane soluble fraction obtained from the methanol extract exhibited marked inhibitory activity from which were isolated two delta(5,7)-sterols (ergosterol and 7-dehydroporiferasterol), two delta(5,7,9(11))-sterols [7,9(11)-dehydroergosterol and 7,9(11)-bisdehydroporiferasterol], two 5 alpha, 8 alpha-epidioxy-delta(6)-sterols (ergosterol peroxide and 7-dehydroporiferasterol peroxide), and a 7-oxo-delta(5)-sterol (7-oxocholesterol), among others. The delta 5'7-sterols, 5 alpha, 8 alpha-epidioxy-delta(6)-sterols and 7-oxo-delta 5-sterol inhibited TPA-induced inflammation in mice. The 50% inhibitory dose of these compounds for TPA-induced inflammation was 0.2-0.7 mg/ear. Furthermore, ergosterol peroxide markedly inhibited the tumor-promoting effect of TPA in 7,12-dimethylbenz[a]anthracene-initiated mice.
Authors	K Yasukawa, T Akihisa, H Kanno, T Kaminaga, M Izumida, T Sakoh, T Tamura, M Takido
Journal	Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 19 Issue 4 Pg. 573-6 (Apr 1996) ISSN: 0918-6158 [Print] Japan
PMID	8860961 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Anticarcinogenic Agents Carcinogens Cell Extracts Sterols 9,10-Dimethyl-1,2-benzanthracene Tetradecanoylphorbol Acetate ergosterol-5,8-peroxide Ergosterol
Topics	9,10-Dimethyl-1,2-benzanthracene (pharmacology) Animals Anticarcinogenic Agents (pharmacology) Carcinogens (antagonists & inhibitors, pharmacology) Cell Extracts (chemistry, pharmacology) Chlorella (chemistry) Chromatography Ergosterol (analogs & derivatives, pharmacology, therapeutic use) Female Inflammation (chemically induced, prevention & control) Mice Mice, Inbred Strains Molecular Structure Skin (drug effects) Skin Diseases (chemically induced, prevention & control) Sterols (chemistry, pharmacology) Tetradecanoylphorbol Acetate (antagonists & inhibitors, pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!