HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

N-formyl-methionyl-leucyl-phenylalanine induces and modulates IL-1 and IL-6 in human PBMC.

Abstract
N-formyl-methionyl-leucyl-phenylalanine (fMLP), a bacterial derivative, induces and modulates various cellular responses linked to inflammation. In this work we evaluated the impact of fMLP stimulation on three pro-inflammatory cytokines: IL-1 alpha, IL-1 beta and IL-6. We found that fMLP induces the secretion of IL-1 alpha, IL-1 beta and IL-6 in human peripheral blood mononuclear cells (PBMC). It also increased LPS-induced secretion of these three cytokines. Northern blot analysis demonstrated that fMLP induced IL-1 alpha, IL-1 beta and IL-6 gene expression by human PBMC. The fMLP-induced IL-1 alpha and IL-1 beta gene expression and IL-6 secretion were abolished by pertussis toxin pretreatment, which suggests that the fMLP induction of cytokine was also mediated via a Gi protein. The concentration range of fMLP used to obtain these effects, in a dose dependent fashion, was 20 microM to 1100 microM. The mechanism by which fMLP modulates cytokine secretion is still not characterized. fMLP seems to share similar biological activities with other chemotactic factors (C5a, MCP-1, PAF, IL-8) that are able to modulate cytokines, and whose receptors belong to the same superfamily as the fMLP receptor(s).
AuthorsN Arbour, P Tremblay, D Oth
JournalCytokine (Cytokine) Vol. 8 Issue 6 Pg. 468-75 (Jun 1996) ISSN: 1043-4666 [Print] England
PMID8818543 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Interleukin-1
  • Interleukin-6
  • Virulence Factors, Bordetella
  • N-Formylmethionine Leucyl-Phenylalanine
  • Pertussis Toxin
Topics
  • Blotting, Northern
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay
  • Gene Expression Regulation (drug effects)
  • Humans
  • Interleukin-1 (genetics, metabolism)
  • Interleukin-6 (metabolism)
  • Kinetics
  • Leukocytes, Mononuclear (drug effects, metabolism)
  • N-Formylmethionine Leucyl-Phenylalanine (pharmacology)
  • Pertussis Toxin
  • Virulence Factors, Bordetella (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: