Abstract |
Testicular tumorigenesis was observed in transgenic mice expressing the 6-kb mouse inhibin alpha-subunit promoter/Simian virus 40 T-antigen (SV40 Tag) fusion gene. The tumors were confined to Leydig cells using immunohistochemistry with anti-Tag antibody, specific binding of biotinylated hCG and histochemistry for 3 beta-hydroxysteroid dehydrogenase. Leydig cell hyperplasia and presence of Tag protein in the testicular interstitial tissue were already evident at 5 and 6.5 days of age, respectively. An immortalized cell line, BLT-1, was established from one testicular tumor. These cells expressed the LH receptor and P450scc mRNAs, and displayed LH-responsive cAMP and progesterone production, and low testosterone production. The cells also specifically bound 125I-labeled recombinant human LH with high affinity (36000 binding sites/cell), and the binding was regulated by 8Br-cAMP and hCG. This gonadal tumor model is valuable for further studies on endocrine functions of Leydig cells and their tumorigenesis in vivo and in vitro.
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Authors | K Kananen, M Markkula, T el-Hefnawy, F P Zhang, T Paukku, J G Su, A J Hsueh, I Huhtaniemi |
Journal | Molecular and cellular endocrinology
(Mol Cell Endocrinol)
Vol. 119
Issue 2
Pg. 135-46
(May 31 1996)
ISSN: 0303-7207 [Print] Ireland |
PMID | 8807633
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antigens, Polyomavirus Transforming
- Chorionic Gonadotropin
- Peptides
- RNA, Messenger
- Receptors, LH
- inhibin-alpha subunit
- 8-Bromo Cyclic Adenosine Monophosphate
- Testosterone
- Progesterone
- Inhibins
- Luteinizing Hormone
- Cyclic AMP
- 3-Hydroxysteroid Dehydrogenases
- Cholesterol Side-Chain Cleavage Enzyme
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Topics |
- 3-Hydroxysteroid Dehydrogenases
(metabolism)
- 8-Bromo Cyclic Adenosine Monophosphate
(pharmacology)
- Animals
- Antigens, Polyomavirus Transforming
(genetics, physiology)
- Cell Line, Transformed
- Cholesterol Side-Chain Cleavage Enzyme
(genetics)
- Chorionic Gonadotropin
(metabolism, pharmacology)
- Cyclic AMP
(analysis)
- Humans
- Hyperplasia
- Inhibins
- Leydig Cell Tumor
(etiology, pathology, physiopathology)
- Leydig Cells
(pathology)
- Luteinizing Hormone
(metabolism)
- Male
- Mice
- Mice, Transgenic
- Peptides
(genetics)
- Progesterone
(analysis)
- Promoter Regions, Genetic
(genetics)
- RNA, Messenger
(analysis)
- Receptors, LH
(analysis, genetics)
- Simian virus 40
(immunology)
- Testicular Neoplasms
(etiology, pathology)
- Testosterone
(analysis)
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