Abstract |
In order to improve the deposition of mitomycin C (MMC) in the administered site, water-insoluble mitomycin C- albumin conjugate (MMC-G-BSA) was prepared. MMC was covalently attached to the glutarylated bovine serum albumin (G-BSA) in the presence of 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide hydrochloride (EDC) to give MMC-G-BSA. The MMC content of the conjugate (16.3% (w/w)) was higher than that of water-soluble mitomycin C- albumin conjugates. MMC was liberated from MMC-G-BSA suspended in a phosphate buffer (pH 7.4, 37 degrees C) with a half-life of 155.3 h. In the same buffer system containing alpha-chymotrypsin, MMC-G-BSA was dissolved perfectly within 24 h due to enzymatic degradation, and the liberation of MMC from the conjugate was significantly accelerated (t1/2 = 24.5 h). After intraperitoneal injection in mice, most of the MMC-G-BSA was retained in the abdominal cavity. Furthermore, the survival time of mice inoculated with Sarcoma 180 was significantly increased by the intraperitoneal injection of MMC-G-BSA. These findings suggest that MMC-G-BSA is a biodegradable macromolecular hybrid which acts as a sustained release delivery system of MMC.
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Authors | T Tanaka, Y Kaneo, M Miyashita, S Shiramoto |
Journal | Biological & pharmaceutical bulletin
(Biol Pharm Bull)
Vol. 18
Issue 12
Pg. 1724-8
(Dec 1995)
ISSN: 0918-6158 [Print] Japan |
PMID | 8787796
(Publication Type: Journal Article)
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Chemical References |
- Albumins
- Antibiotics, Antineoplastic
- Delayed-Action Preparations
- Mitomycin
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Topics |
- Albumins
(administration & dosage, chemistry)
- Animals
- Antibiotics, Antineoplastic
(administration & dosage)
- Delayed-Action Preparations
- Mice
- Mitomycin
(administration & dosage, chemistry)
- Sarcoma 180
(drug therapy)
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