In this study, we sought to determine whether liposomal preparations containing a
phospholipid conjugate of
methotrexate and dimyristoylphosphatidylethanolamine (
MTX-gamma-DMPE) incorporated within their
lipid membranes are effective in suppressing established joint
inflammation in a monoarticular model of
arthritis in the rat.
Arthritis was induced in the right knee joint of Lewis rats. The rats were treated with a single
intra-articular injection of either free
methotrexate (MTX), liposomal MTX [MTX-multilamellar vesicles (MLV)-1.2 microns or MTX-small
unilamellar vesicles (SUV)-100 nm], control
liposomes (E-LIPO) or saline into the inflamed knee 7 days after
arthritis induction. There was no significant difference in knee swelling in MTX-, saline- and E-LIPO-treated rats up to 21 days
after treatment. However, MTX-MLV treatment produced a significant reduction in knee swelling (26.5 +/- 6.0%: mean +/- S.E.M.) 1 day after
intra-articular injection compared with MTX (3.5 +/- 3.5%) and MTX-SUV (14.4 +/- 2.4%), respectively. Over the next 20 days, knee swelling in MTX-MLV-treated rats fell progressively and almost returned to normal. MTX-MLV treatment also inhibited the cellular infiltration associated with the
arthritis. Large multilamellar liposomal preparations of
MTX-gamma-DMPE are more effective than free MTX and MTX-SUV in suppressing
inflammation. Their differential effects in treating the
antigen-induced
arthritis model are related to their retention within the joint space.