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A single intra-articular injection of liposomally conjugated methotrexate suppresses joint inflammation in rat antigen-induced arthritis.

Abstract
In this study, we sought to determine whether liposomal preparations containing a phospholipid conjugate of methotrexate and dimyristoylphosphatidylethanolamine (MTX-gamma-DMPE) incorporated within their lipid membranes are effective in suppressing established joint inflammation in a monoarticular model of arthritis in the rat. Arthritis was induced in the right knee joint of Lewis rats. The rats were treated with a single intra-articular injection of either free methotrexate (MTX), liposomal MTX [MTX-multilamellar vesicles (MLV)-1.2 microns or MTX-small unilamellar vesicles (SUV)-100 nm], control liposomes (E-LIPO) or saline into the inflamed knee 7 days after arthritis induction. There was no significant difference in knee swelling in MTX-, saline- and E-LIPO-treated rats up to 21 days after treatment. However, MTX-MLV treatment produced a significant reduction in knee swelling (26.5 +/- 6.0%: mean +/- S.E.M.) 1 day after intra-articular injection compared with MTX (3.5 +/- 3.5%) and MTX-SUV (14.4 +/- 2.4%), respectively. Over the next 20 days, knee swelling in MTX-MLV-treated rats fell progressively and almost returned to normal. MTX-MLV treatment also inhibited the cellular infiltration associated with the arthritis. Large multilamellar liposomal preparations of MTX-gamma-DMPE are more effective than free MTX and MTX-SUV in suppressing inflammation. Their differential effects in treating the antigen-induced arthritis model are related to their retention within the joint space.
AuthorsA S Williams, J P Camilleri, R M Goodfellow, B D Williams
JournalBritish journal of rheumatology (Br J Rheumatol) Vol. 35 Issue 8 Pg. 719-24 (Aug 1996) ISSN: 0263-7103 [Print] England
PMID8761182 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antirheumatic Agents
  • Drug Carriers
  • Liposomes
  • Phosphatidylethanolamines
  • Serum Albumin, Bovine
  • Methotrexate
  • 1,2-dimyristoylphosphatidylethanolamine
Topics
  • Animals
  • Antirheumatic Agents (administration & dosage, pharmacokinetics)
  • Arthritis (chemically induced, complications, prevention & control)
  • Drug Carriers
  • Injections, Intra-Articular
  • Liposomes
  • Male
  • Methotrexate (administration & dosage, pharmacokinetics)
  • Phosphatidylethanolamines (administration & dosage, pharmacokinetics)
  • Rats
  • Rats, Inbred Lew
  • Serum Albumin, Bovine
  • Severity of Illness Index
  • Time Factors
  • Treatment Outcome

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