Active expression of G gamma globin gene on chromosome 11 with Yunnanese A gamma delta beta)0-thalassemia deletion in MEL cells.

Abstract

A permanent lymphocyte cell line of a heterozygote with Yunnanese (A gamma delta beta)0-thalassemia deletion, associated with an increased production of G gamma globin in adult, was founded using Epstein-Barr virus transformation. The hybrids of the lymphocyte cell and mouse erythroleukemia cell (MEL) were achieved and the hybrids containing human chromosome 11 were selected with the monoclonal antibody 53/6. The subclones containing only either the normal or the abnormal human chromosome 11 were separated and the expression of the human globin genes was studied. Expression of the beta-globin gene, but not the G gamma and A gamma, was observed in the hybrids containing only the normal human chromosome 11, while active expression of the G gamma globin gene was observed in the hybrids containing only the abnormal human chromosome 11. These results have confirmed that the DNA deletion in the beta-globin gene cluster is the cause of persistent active expression of the G gamma globin gene in the Yunnanese mutant.

• Authors: J Zhang, J Qiao, W Song, Z Qiu
• Journal: Science in China. Series C, Life sciences (Sci China C Life Sci) Vol. 39 Issue 3 Pg. 329-36 (Jun 1996) ISSN: 1006-9305 [Print] China
• PMID: 8760459 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Globins

Topics

• Adult
• Animals
• Cell Fusion
• Cell Line
• Chromosomes, Human, Pair 11
• Gene Deletion
• Gene Expression
• Globins (genetics)
• Humans
• Leukemia, Experimental (pathology)
• Male
• Mice
• Thalassemia (genetics, pathology)
• Tumor Cells, Cultured