The proliferation index of MIB-1 as a prognostic factor for patients with transitional cell carcinoma of the upper urinary tract.

Abstract

BACKGROUND:

Recently, several reports have shown that immunohistochemical analysis using MIB-1 antibody, which recognizes Ki-67 (a human nuclear antigen expressed of proliferating cells), is a useful method for determining the proliferative activity of various cancers. In this study, the authors evaluated the prognostic usefulness of the proliferation index using MIB-1 antibody in transitional cell carcinoma of the upper urinary tract.

METHODS:

Proliferation activity was investigated immunohistochemically using monoclonal antibody MIB-1 in formalin fixed, paraffin embedded tissues obtained from 67 specimens of renal pelvic and ureteral cancer. The MIB-1 proliferation index values were calculated from each sample as the percentage of positive nuclei expressed in tumor cells and the clinicopathologic correlation evaluated.

RESULTS:

The MIB-1 proliferation index values were correlated with prognostic parameters such as pathologic stage ( < or = pT1 vs. > or = pT2, P < 0.0005), histologic grade (G1 vs. G2, P < 0.01; G1 vs. G3, P < 0.0001; G2 vs. G3, P < 0.001), and prognosis (P < 0.0001). When patients were subgrouped using index values, patients with higher indices ( > or = 24%) had significantly poorer survival (P < 0.0001). This was especially observed in the G2 group, in which 9 of 10 patients in the higher indices subgroup had a high incidence of recurrence and died. In contrast, only 2 of 29 patients in the lower indices subgroup died. The higher indices subgroup had significantly worse cause specific survival (P < 0.0001). Furthermore, with regard to the muscle invasive tumors ( > or = pT2), the higher indices subgroup also had significantly worse cause specific survival (P < 0.0001).

CONCLUSIONS:

The results of the evaluation of prognostic parameters indicate that the MIB-1 proliferation index is a useful prognostic factor and may enhance the accuracy of conventional morphologic grading and pathologic staging systems.

• Authors: G M Chowdhury, K Kojima, H Kanayama, M Tsuji, Y Kurokawa, S Kagawa
• Journal: Cancer (Cancer) Vol. 78 Issue 4 Pg. 827-33 (Aug 15 1996) ISSN: 0008-543X [Print] United States
• PMID: 8756378 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Biomarkers, Tumor
• Ki-67 Antigen
• Neoplasm Proteins
• Nuclear Proteins

Topics

• Adult
• Aged
• Aged, 80 and over
• Antibodies, Monoclonal
• Biomarkers, Tumor (analysis, immunology)
• Carcinoma, Transitional Cell (chemistry, pathology)
• Cell Division (physiology)
• Evaluation Studies as Topic
• Female
• Humans
• Immunohistochemistry
• Ki-67 Antigen
• Male
• Middle Aged
• Multivariate Analysis
• Neoplasm Proteins (analysis, immunology)
• Neoplasm Staging
• Nuclear Proteins (analysis, immunology)
• Prognosis
• Urologic Neoplasms (chemistry, pathology)