Immunohistochemical characterization of canine transmissible venereal tumor.

Abstract	The collective immunohistochemical expression of human lysozyme, human alpha-1-antitrypsin, human CD3 antigen, calf vimentin, human keratins, human lambda light chains,canine immunoglobulins IgG, IgM, and bovine protein S-100 has been analyzed on formalin-fixed, paraffin-embedded tissue sections of 25 spontaneous canine transmissible venereal tumors (CTVT) from both genital and extragenital locations using the avidin-biotin-peroxidase complex technique. Lysozyme immunoreactivity was detected in 10/25 CTVT, alpha-1-antitrypsin in 14/25 CTVT, and vimentin in 25/25 CTVT. All CTVT cells were negative to keratins 5 + 8 of the Moll catalogue (RCK-102), S-100 protein, lambda light-chain immunoglobulins, IgG, IgM, and CD3 antigen. The intratumoral T-and B-lymphocyte infiltrate was differentiated using CD3 antigen, lambda light-chain immunoglobulins, IgG, and IgM, and this technique could be useful to evaluate the regressive or progressive growth stage of venereal tumors. Our findings support the hypothesis of a histiocytic immunophenotype for CTVT, and these staining techniques could be used in the differential diagnosis with lymphomas.
Authors	E Mozos, A Méndez, J C Gómez-Villamandos, J Martín De Las Mulas, J Pérez
Journal	Veterinary pathology (Vet Pathol) Vol. 33 Issue 3 Pg. 257-63 (May 1996) ISSN: 0300-9858 [Print] United States
PMID	8740698 (Publication Type: Journal Article)
Chemical References	CD3 Complex Immunoglobulin G Immunoglobulin M S100 Proteins Vimentin alpha 1-Antitrypsin Keratins Muramidase
Topics	Animals CD3 Complex (analysis, metabolism) Cattle Diagnosis, Differential Dog Diseases (metabolism, pathology) Dogs Female Humans Immunoglobulin G (analysis, metabolism) Immunoglobulin M (analysis, metabolism) Immunohistochemistry (methods) Keratins (analysis, metabolism) Male Muramidase (analysis, metabolism) S100 Proteins (analysis, metabolism) Venereal Tumors, Veterinary (chemistry, metabolism, pathology) Vimentin (analysis, metabolism) alpha 1-Antitrypsin (analysis, metabolism)

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