A biological method for the quantitative measurement of tetrodotoxin (TTX): tissue culture bioassay in combination with a water-soluble tetrazolium salt.

Abstract

A tissue culture bioassay, using the mouse neuroblastoma cell line (Neuro2A), was improved to provide a simple and sensitive bioassay for TTX or sodium channel-blocking toxins (SCB). The water-soluble tetrazolium salt, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetraz olium, monosodium salt (WST-1), was applied to replace the time-consuming and subjective cell-counting procedure of the cells with automatic measurement, using a microplate reader. It was also confirmed that this method is directly applicable to bacterial culture supernatants, with the precaution of possible interference.

• Authors: K Hamasaki, K Kogure, K Ohwada
• Journal: Toxicon : official journal of the International Society on Toxinology (Toxicon) Vol. 34 Issue 4 Pg. 490-5 (Apr 1996) ISSN: 0041-0101 [Print] England
• PMID: 8735249 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Tetrazolium Salts
• Water
• Tetrodotoxin

Topics

• Animals
• Biological Assay
• Culture Techniques
• Dose-Response Relationship, Drug
• Escherichia coli (metabolism)
• Mice
• Neuroblastoma (pathology)
• Reproducibility of Results
• Solubility
• Tetrazolium Salts (chemistry)
• Tetrodotoxin (analysis, metabolism)
• Tumor Cells, Cultured
• Water