Butyrate enema therapy stimulates mucosal repair in experimental colitis in the rat.

Abstract

BACKGROUND:

The short chain fatty acid (SCFA) butyrate provides energy for colonocytes, stimulates colonic fluid and electrolyte absorption and is recognised as an effective treatment for multiple types of colitis.

AIM:

To examine the impact of butyrate enema therapy on the clinical course, severity of inflammation, and SCFA stimulated Na+ absorption in a chronic experimental colitis.

METHODS:

Distal colitis was induced in rats with a trinitrobenzenesulphonic acid (TNBS) enema. Five days after induction, rats were divided into groups to receive: no treatment, saline enemas, or 100 mM Na-butyrate enemas daily. On day 24, colonic damage score and tissue myeloperoxidase (MPO) activity were evaluated. Colon was mounted in Ussing chambers and Na+ transport and electrical activities were measured during a basal period and after stimulation with 25 mM butyrate.

RESULTS:

In the untreated and the saline enema treated TNBS groups, diarrhoea and extensive colonic damage were seen, associated with increased tissue MPO activities and absent butyrate stimulated Na+ absorption. In contrast, in the butyrate enema treated TNBS group, diarrhoea ceased, colonic damage score improved, and tissue MPO activity as well as butyrate stimulated Na+ absorption recovered to control values.

CONCLUSION:

Butyrate enema therapy stimulated colonic repair, as evidenced by clinical recovery, decreased inflammation, and restoration of SCFA stimulated electrolyte absorption.

• Authors: J D Butzner, R Parmar, C J Bell, V Dalal
• Journal: Gut (Gut) Vol. 38 Issue 4 Pg. 568-73 (Apr 1996) ISSN: 0017-5749 [Print] England
• PMID: 8707089 (Publication Type: Clinical Trial, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)

Chemical References

• Butyrates
• Trinitrobenzenesulfonic Acid
• Peroxidase

Topics

• Animals
• Butyrates (administration & dosage, therapeutic use)
• Colitis (chemically induced, metabolism, pathology, therapy)
• Colon (metabolism)
• Disease Models, Animal
• Enema
• Ion Transport (drug effects)
• Male
• Mucous Membrane (metabolism)
• Peroxidase (metabolism)
• Rats
• Rats, Wistar
• Trinitrobenzenesulfonic Acid