Abstract |
The aim of the study was to use a virus-free system to transfer the Herpes Simplex Virus- thymidine kinase (HSV-TK) gene in mice bearing melanoma tumours. B16 F1 murine melanoma cells were injected subcutaneously. On days 11 and 14, an intratumoral injection of either naked plasmid containing the HSV-TK gene (pAG0) or pAG0-lipofectamine complexes was given. Ganciclovir (120 mg/kg/day) was given for 5 days starting on day 14. Tumour weight reduction (40-50%) was observed in treated animals versus different control groups. Moreover, histopathological analysis on tumours showed large areas of cavitary necrosis (85%) in treated groups compared to controls (10%). Using a simple and safe method, the results presented here demonstrated that virus-free mediated delivery of the HSV-TK gene is efficient in vivo in murine malignant melanoma.
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Authors | C Soubrane, R Mouawad, O Rixe, V Calvez, A Ghoumari, O Verola, M Weil, D Khayat |
Journal | European journal of cancer (Oxford, England : 1990)
(Eur J Cancer)
Vol. 32A
Issue 4
Pg. 691-5
(Apr 1996)
ISSN: 0959-8049 [Print] England |
PMID | 8695274
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Viral Structural Proteins
- Thymidine Kinase
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Topics |
- Animals
- Gene Transfer Techniques
- Genes, Viral
(genetics)
- Genetic Vectors
- Male
- Melanoma, Experimental
(genetics, therapy)
- Mice
- Mice, Inbred C57BL
- Simplexvirus
(genetics)
- Thymidine Kinase
(genetics, therapeutic use)
- Viral Structural Proteins
(genetics)
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