Efficacy of perfluorocarbon partial liquid ventilation in a large animal model of acute respiratory failure.

Abstract

OBJECTIVE:

To demonstrate the efficacy of partial perfluorocarbon liquid ventilation in large animal model of acute respiratory failure.

DESIGN:

Prospective, randomized, controlled trial.

SETTING:

Animal laboratory at a university medical center.

SUBJECTS:

Ten adult sheep, weighing 53.0 +/- 2.8 kg.

INTERVENTIONS:

After assessment of baseline physiologic data, acute respiratory failure was induced by right atrial injection of oleic acid (0.2 mL/kg). Five animals (partial liquid ventilation group) underwent sequential intratracheal dosing of 10 mL/kg of perflubron at 30-min intervals to the following cumulative doses: 10, 20, 30, 40, and 50 mL/kg. The remaining five animals were gas ventilated (control group). Physiologic data were assessed at 30-min intervals in both groups for the 2.5-hr experimental period or until death.

MEASUREMENTS AND MAIN RESULTS:

When compared with control animals, intratracheal perfluorocarbon instillation resulted in significant improvements in arterial oxygen saturation (arterial oxygen saturation after 50 mL/kg: partial liquid ventilation, 96 +/- 3%; control, 55 +/- 8%; p = .001) and physiologic shunt (physiologic shunt after 50 mL/kg dose: partial liquid ventilation, 2 +/- 8%; control, 64 +/- 5%; p = .004). Oxygen delivery improved with perfluorocarbon instillation, but this improvement was not significant. No significant difference in pulmonary compliance was observed during partial liquid ventilation when compared with controls (pulmonary compliance: partial liquid ventilation, 0.43 +/- 0.04 mL/ cm H2O/kg; control, 0.53 +/- 0.03 mL/cm H2O/kg; p = .102).

CONCLUSIONS:

Partial liquid ventilation with perflubron provides effective improvement in gas exchange in an adult animal model of respiratory failure.

• Authors: M C Overbeck, T Pranikoff, C M Yadao, R B Hirschl
• Journal: Critical care medicine (Crit Care Med) Vol. 24 Issue 7 Pg. 1208-14 (Jul 1996) ISSN: 0090-3493 [Print] United States
• PMID: 8674337 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Fluorocarbons

Topics

• Acute Disease
• Administration, Inhalation
• Animals
• Disease Models, Animal
• Fluorocarbons (administration & dosage)
• Prospective Studies
• Random Allocation
• Respiration, Artificial
• Respiratory Distress Syndrome (drug therapy)
• Respiratory Insufficiency (drug therapy)
• Sheep