Abstract |
Endometrial lymphomyeloid cell subsets were evaluated in samples from normal women and from women with abnormal uterine bleeding due to subcutaneous levonorgestrel implants ( Norplant) or an intrauterine device (IUD). The frequency of CD3(+), CD68(+), CD43(+) and endometrial granulated lymphoid cells was evaluated by immunohistochemical or phloxine- tartrazine staining of formalin-fixed paraffin-embedded samples. In normal women, cyclic variation in lymphomyeloid subsets was seen. In women using Norplant for contraception, the frequency of CD3(+), CD68(+) and CD43(+) cells was dramatically decreased, compatible with endometrial atrophy. When Norplant users with abnormal bleeding were compared to women without bleeding, however, the number of CD68(+) cells was significantly increased and the number of CD3(+) and CD43(+) cells was preserved, contrary to the hypothesis that this group would show a greater degree of atrophy and hence, tissue fragility. A similar pattern was seen in a preliminary study of women with bleeding associated with use of copper-only IUD contraception, and in samples taken from late secretory and menstrual biopsies from normal cycling women. Whether these changes in endometrial lymphomyeloid cells represent a result of bleeding arising from a common mechanism or rather cause the uterine bleeding is discussed.
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Authors | D A Clark, S Wang, P Rogers, G Vince, B Affandi |
Journal | Human reproduction (Oxford, England)
(Hum Reprod)
Vol. 11
Issue 7
Pg. 1438-44
(Jul 1996)
ISSN: 0268-1161 [Print] England |
PMID | 8671483
(Publication Type: Journal Article)
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Chemical References |
- Contraceptive Agents, Female
- Drug Implants
- Levonorgestrel
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Topics |
- Adult
- Contraceptive Agents, Female
(administration & dosage, adverse effects)
- Drug Implants
- Endometrium
(drug effects, pathology)
- Female
- Flow Cytometry
- Humans
- Immunohistochemistry
- Intrauterine Devices
(adverse effects)
- Leukocytes
(classification, immunology, pathology)
- Levonorgestrel
(administration & dosage, adverse effects)
- Middle Aged
- Phenotype
- Uterine Hemorrhage
(chemically induced, etiology, pathology)
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