Abstract |
To study cow's milk allergy at the cellular level, we assessed the reactivity of peripheral blood mononuclear cells from patients allergic to cow's milk to alpha s1-casein, which is one of the major allergens in cow's milk. Proliferation of the cells to alpha s1-casein activation showed a rather weak response. Therefore to understand T-cell reactivity to alpha s1-casein in more detail, we prepared alpha s1-casein-specific T-cell lines from patients allergic to cow's milk and established 26 T-cell lines. These T-cell lines could be classified into three groups by analyzing their surface marker expression: those containing predominantly CD4+ CD8- T cells, those containing both CD4+CD8- and CD4-CD8+ T cells, and those containing predominantly CD4- CD8+ T cells. The CD8+ T cells were obtained at an unexpectedly higher frequency from the patients. These T-cell lines produced interferon-gamma and IL-4. These results suggest that CD8+ T cells specific for alpha s1-casein and CD4+ T cells were primed by the stimulation with alpha s1-casein in patients allergic to milk and that both T cells may play a key role in the onset, progression of, or recovery from cow's milk allergy.
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Authors | H Nakajima, S Hachimura, S Nishiwaki, T Katsuki, N Shimojo, A Ametani, Y Kohno, S Kaminogawa |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 97
Issue 6
Pg. 1342-9
(Jun 1996)
ISSN: 0091-6749 [Print] United States |
PMID | 8648031
(Publication Type: Journal Article)
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Chemical References |
- Caseins
- Cytokines
- Interleukin-4
- Interferon-gamma
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Topics |
- CD8-Positive T-Lymphocytes
(immunology)
- Caseins
(immunology)
- Cells, Cultured
- Child, Preschool
- Cytokines
(metabolism)
- Dermatitis, Atopic
(immunology)
- Humans
- Interferon-gamma
(biosynthesis)
- Interleukin-4
(biosynthesis)
- Lymphocyte Activation
- Milk Hypersensitivity
(immunology)
- T-Lymphocytes
(immunology)
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