Changes in cellular components of spleen and lymph node cells and the effector cells responsible for Meth A tumor eradication induced by zinostatin stimalamer.

Abstract

We reported previously that pretreatment with zinostatin stimalamer (ZSS) eradicated Meth A tumors in BALB/c mice. We herein investigated cellular components of spleen and lymph node cells of Meth A-bearing ZSS-pretreated mice by flow cytometry; the antitumor effector cells by in vivo depletion of T cells, NK cells, or macrophages; and host-mediated antitumor activity associated with ZSS treatment after tumor transplantation. ZSS given on day-3 transiently decreased the number of spleen cells. The percentage of T cells increased, but B cells and macrophages decreased. B cells decreased in inguinal lymph nodes in Meth A-bearing ZSS-pretreated mice, but increased in Meth A-bearing control mice. In vivo depletion experiments using antibodies or carrageenan showed that antitumor effector cells for tumor eradication are Thy1.2+/Lyt2.2+ and that at least a part of them are asialo GM1+. Thy1.2+/Lyt2.2+/asialoGM1- cells are important in generation of the antitumor activity of ZSS; however, L3T4+ T cells are also involved in initiation of tumor eradication. The result of ZSS treatment after tumor transplantation suggests that ZSS might exhibit antitumor activity by augementating host-mediated antitumor resistance, as well as its intrinsic cytocidal activity.

• Authors: E Masuda, H Maeda
• Journal: Cancer research (Cancer Res) Vol. 56 Issue 8 Pg. 1868-73 (Apr 15 1996) ISSN: 0008-5472 [Print] United States
• PMID: 8620506 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antigens, Ly
• Maleic Anhydrides
• Polystyrenes
• Thy-1 Antigens
• poly(maleic acid-styrene)neocarzinostatin
• Zinostatin

Topics

• Animals
• Antibodies, Monoclonal
• Antigens, Ly (analysis)
• B-Lymphocytes (drug effects, immunology, pathology)
• Female
• Fibrosarcoma (drug therapy, immunology, pathology)
• Immunophenotyping
• Killer Cells, Natural (drug effects, immunology, pathology)
• Lymph Nodes (drug effects, immunology, pathology)
• Lymphocyte Depletion
• Macrophages (drug effects, immunology, pathology)
• Maleic Anhydrides (therapeutic use)
• Mice
• Mice, Inbred BALB C
• Polystyrenes (therapeutic use)
• Spleen (drug effects, immunology, pathology)
• T-Lymphocytes (drug effects, immunology, pathology)
• Thy-1 Antigens (analysis)
• Time Factors
• Zinostatin (analogs & derivatives, therapeutic use)