Abstract |
We studied characteristics of the anti-glomerular basement membrane (GBM) antibody response in three animal models of Goodpasture's disease: treatment of Brown Norway (BN) rats with the polyclonal activator mercuric chloride ( HgCl2), and immunization of BN and Wistar Kyoto (WKY) rats with rat GBM. Serial serum samples were obtained over the time course of the models, and anti-GBM antibodies eluted from the kidneys. Functional affinity of the anti-GBM antibodies was measured in a solid-phase ELISA incorporating the mild chaotropic agent diethylamine. Evidence for shared epitope specificity with human anti-GBM antibodies was sought using competition ELISA. As with recent studies in human anti-GBM disease, there was no evidence for affinity maturation of the anti-GBM response in the serum of any of the animal models. Antibodies eluted from the kidneys were of higher functional affinity than serum antibodies only in the HgCl2-treated BN rat. There was no obvious correlation between the functional affinity of the antibodies and the severity of nephritis in the three models. Competition studies between eluted anti-GBM antibodies from the rat models and human anti-GBM antibodies did not provide any evidence for shared epitope recognition. This study provides further information on the extent to which these models reflect the human disease.
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Authors | J B Marriott, F Qasim, J Reynolds, C D Pusey, D B Oliveira |
Journal | Experimental nephrology
(Exp Nephrol)
1995 Jul-Aug
Vol. 3
Issue 4
Pg. 257-64
ISSN: 1018-7782 [Print] Switzerland |
PMID | 8590039
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies
- Autoantibodies
- Dimethylamines
- antiglomerular basement membrane antibody
- Mercuric Chloride
- dimethylamine
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Topics |
- Animals
- Antibodies
(immunology)
- Antibody Affinity
- Antibody Specificity
- Autoantibodies
(blood, immunology)
- Basement Membrane
(immunology)
- Binding, Competitive
- Dimethylamines
(pharmacology)
- Disease Models, Animal
- Enzyme-Linked Immunosorbent Assay
- Glomerulonephritis
(immunology)
- Humans
- Immunization
- Kidney Glomerulus
(immunology)
- Kinetics
- Mercuric Chloride
(immunology, pharmacology)
- Rats
- Rats, Inbred BN
- Rats, Inbred WKY
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