[The outlook for improving the immunoglobulin preparations for the detection and identification of the causative agents of glanders and melioidosis].

Abstract

The paper summarizes the data concerning the production and study of monoclonal antibodies (MAb) to the diagnostically significant glanders and melioidosis bacillus antigens. It evaluates the efficiency of using MAb in the gel immunodiffusion and agglutination tests as a basis of new-generation preparations for fluorescent antibody assay, indirect hemagglutination test which are used while detecting and identifying pathogenic pseudomonads. The paper defines the quality indices for monoclonal luminescent immunoglobulins and provides evidence for the benefits of monoclonal diagnostic agents over polyclonal analogues.

• Authors: N P Khrapova, N G Tikhonov, E V Prokhvatilova, M Ia Kulakov
• Journal: Meditsinskaia parazitologiia i parazitarnye bolezni (Med Parazitol (Mosk)) 1995 Oct-Dec Issue 4 Pg. 49-53 ISSN: 0025-8326 [Print] Russia (Federation)
• Vernacular Title: Perspektivy sovershenstvovaniia immunoglobulinovykh preparatov dlia obnaruzheniia i identifikatsii vozbuditeleĭ sapa i melioidoza.
• PMID: 8587520 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antibodies, Monoclonal
• Immunoglobulins
• Indicators and Reagents

Topics

• Agglutination Tests (methods, standards, statistics & numerical data)
• Animals
• Antibodies, Monoclonal (isolation & purification)
• Burkholderia pseudomallei (immunology, isolation & purification)
• Hybridomas (immunology)
• Immunodiffusion (methods, standards, statistics & numerical data)
• Immunoglobulins (isolation & purification)
• Indicators and Reagents (standards)
• Mice
• Mice, Inbred BALB C
• Pseudomonas (immunology, isolation & purification)