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Evidence of subtype-specific antibodies to antigenic epitopes in the NS5 region of hepatitis C virus in the circulation of patients with chronic hepatitis C.

Abstract
The antigenicity of the NS5 region of type 1 hepatitis C virus (HCV) was investigated by epitope mapping of the region spanning amino acids 2530 to 2723 of the HCV polyprotein. Six antigenic regions were recognized by anti-HCV positive sera from individuals with chronic hepatitis C in a modified enzyme-linked immunosorbent assay with synthetic oligopeptides. Five of these regions demonstrated intra-type 1 sequence variations defining subtype 1a and 1b HCV sequences. The region between amino acids 2623 and 2634 allowed testing of subtype-specific anti-NS5 antibodies; serological reactivity to subtypes 1a and 1b was observed in 27 and 61%, respectively, of 150 cases with chronic hepatitis C. Simultaneous reactivity to subtypes 1a and 1b was found in 23% of the patients. Detection of subtype-specific anti-NS5 antibody correlated in more than 80% of the cases with the HCV genotype (subtypes 1a and 1b) analyzed by PCR amplification of the NS5 sequence. These data provide evidence of the existence of a subtype-specific anti-NS5 response in the circulation of patients with chronic hepatitis C.
AuthorsJ A Quiroga, J Martin, M Pernas, M Pardo, M Herrero, I Castillo, J Bartolome, V Carreño
JournalClinical and diagnostic laboratory immunology (Clin Diagn Lab Immunol) Vol. 1 Issue 5 Pg. 545-51 (Sep 1994) ISSN: 1071-412X [Print] United States
PMID8556499 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Epitopes
  • Hepatitis C Antibodies
  • Hepatitis C Antigens
Topics
  • Adolescent
  • Adult
  • Amino Acid Sequence
  • Antibody Specificity
  • Base Sequence
  • Chronic Disease
  • Cohort Studies
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes
  • Female
  • Genotype
  • Hepacivirus (classification, genetics, immunology)
  • Hepatitis C Antibodies (immunology)
  • Hepatitis C Antigens (immunology, ultrastructure)
  • Humans
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Serotyping

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