Abstract |
The antigenicity of the NS5 region of type 1 hepatitis C virus (HCV) was investigated by epitope mapping of the region spanning amino acids 2530 to 2723 of the HCV polyprotein. Six antigenic regions were recognized by anti-HCV positive sera from individuals with chronic hepatitis C in a modified enzyme-linked immunosorbent assay with synthetic oligopeptides. Five of these regions demonstrated intra-type 1 sequence variations defining subtype 1a and 1b HCV sequences. The region between amino acids 2623 and 2634 allowed testing of subtype-specific anti-NS5 antibodies; serological reactivity to subtypes 1a and 1b was observed in 27 and 61%, respectively, of 150 cases with chronic hepatitis C. Simultaneous reactivity to subtypes 1a and 1b was found in 23% of the patients. Detection of subtype-specific anti-NS5 antibody correlated in more than 80% of the cases with the HCV genotype (subtypes 1a and 1b) analyzed by PCR amplification of the NS5 sequence. These data provide evidence of the existence of a subtype-specific anti-NS5 response in the circulation of patients with chronic hepatitis C.
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Authors | J A Quiroga, J Martin, M Pernas, M Pardo, M Herrero, I Castillo, J Bartolome, V Carreño |
Journal | Clinical and diagnostic laboratory immunology
(Clin Diagn Lab Immunol)
Vol. 1
Issue 5
Pg. 545-51
(Sep 1994)
ISSN: 1071-412X [Print] United States |
PMID | 8556499
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Epitopes
- Hepatitis C Antibodies
- Hepatitis C Antigens
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Topics |
- Adolescent
- Adult
- Amino Acid Sequence
- Antibody Specificity
- Base Sequence
- Chronic Disease
- Cohort Studies
- Enzyme-Linked Immunosorbent Assay
- Epitopes
- Female
- Genotype
- Hepacivirus
(classification, genetics, immunology)
- Hepatitis C Antibodies
(immunology)
- Hepatitis C Antigens
(immunology, ultrastructure)
- Humans
- Male
- Middle Aged
- Molecular Sequence Data
- Polymerase Chain Reaction
- Sensitivity and Specificity
- Serotyping
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