Abstract |
Current diagnosis of melioidosis is based on bacterial culture and/or serology which is becoming increasingly useful. An IgM-ELISA using heat-killed whole cells of Pseudomonas pseudomallei was developed and compared with an indirect haemagglutination technique (IHAT) and an indirect immunofluorescent technique(IFAT). The IgM-ELISA using a P:N ratio of > or = 2 had a sensitivity of 91% and a specificity of 96%. All 3 assays were further used in a seroepidemiological survey amongst different groups of patients and healthy individuals. It was found that the IFAT performed better than the IHAT, detecting antibodies to P. pseudomallei in 6% of diabetics, 5% of pyrexics, 8% of pregnant women and 3% of farmers. For the same groups the IgM-ELISA detected antibodies in 1% of pyrexics, 8% of pregnant women and a further 14% of farmers. The IgM-ELISA was found to be sensitive and useful for the serological diagnosis of acute melioidosis.
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Authors | J Vadivelu, S D Puthucheary, G S Gendeh, N Parasakthi |
Journal | Singapore medical journal
(Singapore Med J)
Vol. 36
Issue 3
Pg. 299-302
(Jun 1995)
ISSN: 0037-5675 [Print] India |
PMID | 8553097
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Adult
- Aged
- Case-Control Studies
- Enzyme-Linked Immunosorbent Assay
(methods)
- Female
- Fluorescent Antibody Technique, Indirect
(methods)
- Hemagglutination Tests
(methods)
- Humans
- Malaysia
(epidemiology)
- Male
- Melioidosis
(blood, epidemiology, immunology, microbiology)
- Middle Aged
- Pregnancy
- Prevalence
- Sensitivity and Specificity
- Seroepidemiologic Studies
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