HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Serodiagnosis of melioidosis in Malaysia.

Abstract
Current diagnosis of melioidosis is based on bacterial culture and/or serology which is becoming increasingly useful. An IgM-ELISA using heat-killed whole cells of Pseudomonas pseudomallei was developed and compared with an indirect haemagglutination technique (IHAT) and an indirect immunofluorescent technique(IFAT). The IgM-ELISA using a P:N ratio of > or = 2 had a sensitivity of 91% and a specificity of 96%. All 3 assays were further used in a seroepidemiological survey amongst different groups of patients and healthy individuals. It was found that the IFAT performed better than the IHAT, detecting antibodies to P. pseudomallei in 6% of diabetics, 5% of pyrexics, 8% of pregnant women and 3% of farmers. For the same groups the IgM-ELISA detected antibodies in 1% of pyrexics, 8% of pregnant women and a further 14% of farmers. The IgM-ELISA was found to be sensitive and useful for the serological diagnosis of acute melioidosis.
AuthorsJ Vadivelu, S D Puthucheary, G S Gendeh, N Parasakthi
JournalSingapore medical journal (Singapore Med J) Vol. 36 Issue 3 Pg. 299-302 (Jun 1995) ISSN: 0037-5675 [Print] India
PMID8553097 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Topics
  • Adult
  • Aged
  • Case-Control Studies
  • Enzyme-Linked Immunosorbent Assay (methods)
  • Female
  • Fluorescent Antibody Technique, Indirect (methods)
  • Hemagglutination Tests (methods)
  • Humans
  • Malaysia (epidemiology)
  • Male
  • Melioidosis (blood, epidemiology, immunology, microbiology)
  • Middle Aged
  • Pregnancy
  • Prevalence
  • Sensitivity and Specificity
  • Seroepidemiologic Studies

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: