Expression of messenger RNA encoding a paraneoplastic cerebellar degeneration-associated antigen in the rat hippocampus.

Abstract

The PCD17 protein is a paraneoplastic cerebellar degeneration (PCD)-associated neural autoantigen which is recognized by autoantibodies in sera of PCD patients. The localization of the PCD17 mRNA was examined by in situ hybridization histochemistry using a PCD17 cRNA probe. In the cerebellum, the localization of the transcript was in agreement with the distribution of PCD17-immunoreactivity. On the other hand, intense hybridization signals were detected in most of cells in the hippocampus in which notable PCD17-immunoreactivity was undetected. These observations suggest that some negative translational or post-transcriptional mechanisms of the PCD17 mRNA may be involved in neurons of the hippocampus.

• Authors: K Sakai, Y Kitagawa, T Ogasawara, G Hirose
• Journal: Neuroscience letters (Neurosci Lett) Vol. 197 Issue 1 Pg. 33-6 (Sep 01 1995) ISSN: 0304-3940 [Print] Ireland
• PMID: 8545049 (Publication Type: Journal Article)

Chemical References

• Autoantigens
• CDR2 protein, human
• Nerve Tissue Proteins
• RNA Probes
• RNA, Messenger
• Recombinant Proteins
• Digoxigenin

Topics

• Animals
• Autoantigens (biosynthesis)
• Cerebellar Diseases (metabolism, pathology)
• Digoxigenin (metabolism)
• Female
• Hippocampus (metabolism)
• Immunohistochemistry
• In Situ Hybridization
• Nerve Degeneration
• Nerve Tissue Proteins (biosynthesis)
• Paraneoplastic Syndromes (metabolism, pathology)
• RNA Probes
• RNA, Messenger (biosynthesis)
• Rats
• Rats, Inbred Lew
• Recombinant Proteins (biosynthesis)